Abstract
Chloramphenicol produces a decrease in the respiratory quotient of dormant and swollenStreptomyces antibioticus spores of about 20–25% and 18–24%, respectively, in the absence of protein synthesis as measured by [3H]leucine incorporation and by chemical methods. Rifampin and streptomycin had no inhibitory effect on respiration, thus excluding the possibility of an effect of all protein synthesis inhibitors on respiration. The inhibition of respiration by chloramphenicol was not a consequence of an increase in mortality due to toxicity, nor was it influenced by the developmental stage of the spores. It seems that chloramphenicol affects the activity of some component(s) related to the electron transport chain ofS. antibioticus spores, situated before the cytochrome oxidase level.
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Literature Cited
Cavari, B., Kalra, V. K., Brodie, A. F. 1971. Oxidative phosphorylation in fractionated bacterial systems. Effect of chloramphenicol. Journal of Bacteriology108:1017–1025.
Dombou, M., Mizuno, T., Mizushina, S. 1977. Interaction of the cytoplasmic membrane and ribosomes inEscherichia coli, altered ribosomal proteins in sucrose-dependent spectinomycin-resistant mutants. Molecular and General Genetics155:53–60.
Freeman, K. B. 1970. Effect of chloramphenicol and its isomers and analogues on the mitochondrial respiratory chain. Canadian Journal of Biochemistry48:469–478.
Freeman, K. B., Haldar, D. 1967. The inhibition of NADH oxidation in mammalian mitochondria by chloramphenicol. Biochemical and Biophysical Research Communications28:8–12.
Freeman, K. B., Haldar, D. 1968. Inhibition of mammalian mitochondrial NADH oxidation by chloramphenicol and its isomers and analogues. Canadian Journal of Biochemistry46:1003–1008.
Hahn, F. E., Wisseman, C. L., Jr., Hopps, H. E. 1955. Mode of action of chloramphenicol. III. Action of chloramphenicol on bacterial energy metabolism. Journal of Bacteriology69:215–223.
Hanson, J. B., Hodges, T. K. 1963. Uncoupling action of chloramphenicol as a basis for the inhibition of ion accumulation. Nature200:1009.
Hanson, J. B., Krueger, W. A. 1966. Impairment of oxidative phosphorylation byd-threo- andl-threo-chloramphenicol. Nature211:1322.
Hardisson, C., Manzanal, M. B., Salas, J. A., Suarez, J. E. 1978. Fine structure, physiology and biochemistry of arthrospore germination inStreptomyces antibioticus. Journal of General Microbiology105:203–214.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry193:265–275.
Mizuno, T., Yamagata, H., Mizushima, S. 1977. Interaction of cytoplasmic membrane and ribosomes inEscherichia coli: Spectinomycin-induced disappearance of membrane protein I–19. Journal of Bacteriology129:326–332.
Turner, G., Lloyd, D. 1971. The effect of chloramphenicol on growth and mitochondrial function of the cilliate protozoonTetrahymena pyriformis strain st. Journal of General Microbiology67:175–188.
Wilson, S. B., Moore, A. L. 1973. The effect of protein synthesis inhibitors on oxidative phosphorylation by plant mitochondria. Biochimica et Biophysica Acta292:603–610.
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Salas, J.A., Hardisson, C. Inhibition of the respiration of dormant and swollenStreptomyces spores by chloramphenicol. Current Microbiology 2, 273–276 (1979). https://doi.org/10.1007/BF02602858
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DOI: https://doi.org/10.1007/BF02602858