Abstract
Southern analysis ofEcoRI-digested BALB/c liver DNA reveals four T-cell receptorTcra-V3-hybridizing DNA fragments, which are of sizes 18.0, 12.0, 8.0, and 2.1 kilobases, respectively. These fourTcra-V3-hybridizing genomic DNA were isolated from a BALB/c genomic library. Restriction and Southern analysis of the genomic DNA clones showed that each of theTcra-V3-hybridizingEco RI DNA fragments harbors only a singleTcra-V3 gene. The DNA sequences of coding regions of the fourTcra-V3 family members were determined. These sequences show very limited divergence from one another. Comparisons ofBALB/c Tcra-V3 sequences with publishedTcra-V3 sequences expressed in different strains of mice reveal substantial allelic polymorphism. Sequence similarity searches retrieved homologous rat, cattle, and human genes. The scarcity of coding sequence divergence among members of theTcra-V3 family and the more substantial allelic polymorphism may be general features of the T-cell receptor V-alpha chain-encoding gene families.
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The nucleotide sequence data reported in this paper have been submitted to the Genbank nucleotide database and have been assigned the accession numbers U26917 (Tcra-V3.1) and U31877-U31879 (Tcra-V3.2–3.4)
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Tan, KN., Huang, K., Myrick, K.V. et al. Diversity of theTcra-V3 gene family in BALB/c mice. Immunogenetics 44, 372–376 (1996). https://doi.org/10.1007/BF02602782
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DOI: https://doi.org/10.1007/BF02602782