Skip to main content
SpringerLink
Log in

Isolation of clones of hamster embryo cells transformed by the bovine papilloma virus

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Primary hamster embryo cells infected with bovine papilloma virus (BPV) or treated with BPV DNA-calcium phosphate precipitates showed striking morphological alterations characteristic of transformed cells. Long, spindle-shaped cells grew into dense foci, eventually overgrowing monolayers of normally shaped cells. Samples of these cells were tested for anchorage-independent growth in dilute agarose medium. Cells were able to grow in agarose to form colonies which, when removed from agarose and transferred to liquid medium, established clones. Mockinfected cultures inoculated with plain medium displayed normal cell morphology and growth properties. This is the first report of BPV-transformed cells demonstrating anchorage-independent growth in agarose and the establishment of BPV transformed clones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Black, P. H., Hartley, J. W., Rowe, W. P., Heubner, R. J. 1963. Transformation of bovine tissue cells by bovine papilloma virus. Nature199:1016–1018.

    Article  PubMed  CAS  Google Scholar 

  2. Boiron, M., Levy, J. P., Thomas, M., Friedmann, J. C., Bernard, J. 1964. Some properties of bovine papilloma virus. Nature201:423–424.

    Article  PubMed  CAS  Google Scholar 

  3. Boiron, M., Thomas, M., Chenaille, P. H. 1965. A biological property of deoxyribonucleic acid extracted from bovine papilloma virus. Virology26:150–153.

    Article  PubMed  CAS  Google Scholar 

  4. Favre, M., Breitburd, F., Croissant, O., Orth, G. 1974. Hemagglutinating activity of bovine papilloma virus. Virology60:572–578.

    Article  PubMed  CAS  Google Scholar 

  5. Geraldes, A. 1969. Malignant transformation of hamster cells by cell-free extracts of bovine papillomas (in vitro). Nature222:1283–1284.

    Article  PubMed  CAS  Google Scholar 

  6. Geraldes, A. 1970. New antigens in hamster embryo cells transformedin vitro by bovine papilloma extracts. Nature226:81–82.

    Article  PubMed  CAS  Google Scholar 

  7. Graham, F. L., van der Eb, A. J. 1973. A new technique for assay of infectivity of human adenovirus 5 DNA. Virology52:456–467.

    Article  PubMed  CAS  Google Scholar 

  8. Law, M.-F., Lancaster, W. D., Howley, P. M. 1979. Conserved polynucleotide sequences among the genome of papillomaviruses. Journal of Virology32:199–207.

    PubMed  CAS  Google Scholar 

  9. Lancaster, W. D. 1979. Physical maps of bovine papillomavirus type 1 and type 2 genomes. Journal of Virology32:684–687.

    PubMed  CAS  Google Scholar 

  10. Lancaster, W. D., Olson, C. 1978. Demonstration of two distinct classes of bovine papilloma virus. Virology89:372–379.

    Article  PubMed  CAS  Google Scholar 

  11. Macpherson, I. 1969. Agar suspension culture for quantitation of transformed cells, pp. 214–219. In: Habel, K., Salzman, N. P. (eds.), Fundamental techniques in virology. New York, San Francisco, London: Academic Press.

    Google Scholar 

  12. Meischke, H. R. C. 1979.In vitro transformation by bovine papilloma virus. Journal of General Virology43:473–487.

    Article  PubMed  CAS  Google Scholar 

  13. Shin, S., Freedman, V. H., Risser, R., Pollack, R. 1975. Tumorigenicity of virus-transformed cells innude mice is correlated specifically with anchorage independent growthin vitro. Proceedings of the National Academy of Sciences of the United States of America72:4435–4439.

    Article  PubMed  CAS  Google Scholar 

  14. Thomas, M., Boiron, M., Tanzer, J., Levy, J. P., Bernard, J. 1964.In vitro transformation of mice cells by bovine papilloma virus. Nature202:709–710.

    Article  PubMed  CAS  Google Scholar 

  15. Thomas, M., Levy, J. P., Tanzer, J., Boiron, M., Bernard, J. 1963.In vitro transformation of embryonal calf skin cells under the action of cell-free extracts of bovine papillomas. Comptes Rendus de l'Académie des Sciences (Paris)257:2155–2158.

    CAS  Google Scholar 

  16. Todaro, G. J., Green, H. 1964. Enhancement by thymidine analogs of susceptibility of cells to transformation by SV40. Virology24:393–400.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Medicine, University of Arizona, 85724, Tucson, Arizona, USA

    Don M. Morgan & William Meinke

Authors
  1. Don M. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William Meinke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morgan, D.M., Meinke, W. Isolation of clones of hamster embryo cells transformed by the bovine papilloma virus. Current Microbiology 3, 247–251 (1980). https://doi.org/10.1007/BF02602457

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02602457

Keywords

Search

Navigation