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Cellular localization of acid carboxypeptidase inAspergillus oryzae

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Abstract

Fresh mycelia ofAspergillus oryzae were shown to have strong acid carboxypeptidase (EC 3.4.12.-) activity. The cellular localization of acid carboxypeptidase was investigated using the broken mycelia and the protoplasts of the fungusAspergillus oryzae IAM 2640. In the broken mycelia, about 40% of the total activity was found in the “cell wall” fraction (2,000×g), with most of the remainder in the soluble fraction (100,000×g supernatant). During the formation of protoplasts, most of the acid carboxypeptidase in the mycelia was solubilized and released. The specific activity of acid carboxypeptidase in the lysed protoplasts was about 10-fold lower than that found in the broken mycelial preparation. These data indicate that most of the acid carboxypeptidase is probably located in the cell surface, which includes the cell wall, the periplasm, and the cell membrane.

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Authors and Affiliations

  1. Laboratory of Enzymology and Microbial Chemistry, Tokyo Nōkō University, Fuchu, 183, Tokyo, Japan

    Takehiko Nomi, Shigeaki Mikami, Akiko Yamane, Michio Takeuchi & Eiji Ichishima

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  1. Takehiko Nomi
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  2. Shigeaki Mikami
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  3. Akiko Yamane
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  4. Michio Takeuchi
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  5. Eiji Ichishima
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Nomi, T., Mikami, S., Yamane, A. et al. Cellular localization of acid carboxypeptidase inAspergillus oryzae . Current Microbiology 1, 25–28 (1978). https://doi.org/10.1007/BF02601702

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