Abstract
Experiments were designed to clarify the role of the brain's organum vasculosum laminae terminalis (OVLT) in the development of fever in rabbits. Rectal and ear skin temperatures were recorded in conscious animals in which the OVLT had been electrolytically destroyed or in which the preoptic anterior hypothalamus (PO/AH) had been transected bilaterally. When the OVLT had been ablated the febrile responses to intravenous injection of interleukin-1 beta (IL-1β) or tumour necrosis factor alpha were significantly attenuated, while those to intracerebroventricular injection of IL-1β were not affected. Fever induced by intracerebroventricular injection of prostaglandin E2 (PGE2) was prolonged significantly. The febrile responses to intravenous injection of IL-1β and to intracerebroventricular injection of PGE2 were attenuated when the transection was located caudally to the anterior wall of the third ventricle and extended laterally more than about 3 mm in the ventricular wall. The results show that the OVLT region is a site through which signals to increase body temperature are transferred from the blood to the brain in rabbits.
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References
Bernadini GL, Richards DB, Lipton JM (1984) Antipyretic effect of centrally administered CRF. Peptides 5:57–59
Blatteis CM, Bealer SL, Hunter WS, Llanos Q-J, Ahokas RA, Mashburn TAJ (1983) Suppression of fever after lesions of the anteroventral third ventricle in guinea pigs. Brain Res Bull 11:519–526
Blatteis CM, Hales JRS, McKinley MJ, Fawcett AA (1987) Role of the anteroventral third ventricle region in fever in sheep. Can J Physiol Pharmacol 65:1255–1260
Camacho A, Phillips MI (1981) Horseradish peroxidase study in rat of the neural connections of the organum vasculosum of the lamina terminalis. Neurosci Lett 25:201–204
Cooper KE (1987) The neurobiology of fever: thoughts on recent developments. Annu Rev Neurosci 10:297–324
Feng JD, Dao T, Lipton JM (1987) Effects of PO/AH microinjections ofα-MSH on fever and normal temperature control in rabbits. Fed Proc 46:682
Hashimoto M (1991) Characterization and mechanism of fever induction by interleuikn-1 beta. Pflügers Arch 419:616–621
Hashimoto M, Bando T, Iriki M, Hashimoto K (1988) Effect of indomethacin on febrile response to recombinant human interleukin 1-alpha in rabbits. Am J Physiol 255:R527-R533
Hashimoto M, Ishikawa Y, Yokota S, Goto F, Bando T, Sakakibara Y, Iriki M (1991) Action site of circulating interleukin-1 on the rabbit brain. Brain Res 540:217–223
Hellon R, Townsend Y, Laburn HP, Mitchell D (1991) Mechanisms of fever. In: Schoenbaum E, Lomax P (eds) Thermoregulation: pathology, pharmacology and therapy. Pergamon New York, pp 19–54
Kluger MJ (1991) Fever: role of pyrogens and cryogens. Physiol Rev 71:93–127
Matsuda T, Hori T, Nakashima T (1992) Thermal and PGE2 sensitivity of the organum vasculosum lamina terminalis region and preoptic area in rat brain slices. J Physiol (Lond) 454:197–212
Matsumura K, Watanabe Y, Imai-Matsumura K, Connolly M, Koyama Y, Onoe H, Watanabe Y (1992) Mapping of prostaglandin E2 binding sites in rat brain using quantative autoradiography. Brain Res 581:292–298
Matsumura K, Watanabe Y, Onoe H, Watanabe Y, Hayaishi O (1990) High density of prostaglandin E2 binding sites in the anterior wall of the 3rd ventricle: a possible site of its hyperthermic action. Brain Res 533:147–151
Milton AS, Wendlandt S (1970) A possible role for prostaglandin E1 as a modulator for temperature regulation in the central nervous system of the cat (abstract) J Physiol (Lond) 207: 76P-77P
Mori S, Goto F, Goto K, Ohkawara S, Maeda S, Shimada K, Yoshinaga M (1988) Cloning and sequence analysis of a cDNA for lymphocyte proliferation potentiating factor of rabbit polymorphonuclear leukocytes: identification rabbit interleukin 1 beta. Biochem Biophys Res Commun 150:1237–1243
Morimoto A, Murakami N, Nakamori T, Watanabe T (1987) Evidence for separate mechanisms of induction of biphasic fever inside and outside the blood-brain barrier in rabbits. J Physiol (Lond) 383:629–637
Morimoto A, Murakami N, Sakata Y, Watanabe T, Yamaguchi K (1990) Functional and structural differences in febrile mechanism between rabbits and rats. J Physiol (Lond) 427:227–239
Nakamori T, Morimoto A, Yamaguchi- K, Watanabe T, Naotoshi M (1994) Interleukin-1β production in the circumventricular organs during endotoxin fever in rabbits. In Plaschka K, Gerstberger R (eds) Integrative and cellular aspects of autonomic functions: temperature and osmoregulation. John Libbey, Paris, pp 153–162
Sawyer CH, Everett W, Green FD (1954) The rabbit diencephalon in stereotaxic coordinates. J Comp Neurol 101:801–824
Stitt JT (1985) Evidence for the involvement of the organum vasculosum laminae terminalis in the febrile response of rabbits and rats. J Physiol (Lond) 368:501–511
Stitt JT (1991) Differential sensitivity in the sites of fever production by prostaglandin E1 within the hypothalamus of the rat. J Physiol (Lond) 432:99–110
Stitt JT, Bernheim HA (1985) Differences in endogenous pyrogen fevers induced by iv and icv routes in rabbits. J Appl Physiol 59:342–347
Stitt JT, Shimada SG (1989) Enhancement of the febrile responses of rats to endogenous pyrogen occurs within the OVLT regiion. J Appl Physiol 67:1740–1746
Strijbos PJ, Hardwick AJ, Relton JK, Carey F, Rothwell NJ (1992) Inhibition of central action of cytokines on fever and thermogenesis by lipocortin-1 involves CRF. Am J Physiol 263:E632-E636
Veale WL, Cooper KE (1974) Evidence for the involovement of prostaglandins in fever. In Lederis K, Veale W (eds) Recent studies of hypothalamic function. Karger, Basel, pp 359–370
Watanabe M (1992) Charactristics of TNFα- and TNFβ-induced fever in the rabbit. Jpn J Physiol 42:101–116
Zelazowski P, Patchev VK, Zelazowska EB, Chrousos GP, Gold PW, Sternberg EM (1993) Release of hypothalamic corticotropin-releasing hormone and arginine-vasopressin by interleukin-1β andα-MSH: studies in rats with different susceptibility to inflammatory disease. Brain Res 631:22–26
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Hashimoto, M., Ueno, T. & Iriki, M. What roles does the organum vasculosum laminae terminalis play in fever in rabbits?. Pflugers Arch. 429, 50–57 (1994). https://doi.org/10.1007/BF02584029
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DOI: https://doi.org/10.1007/BF02584029