Summary
Our experiments were carried outin vitro on different isolated and perfused pancreas preparations: adult rat pancreas, newborn dog pancreas, uncinate process and splenic portion of adult dog pancreas. The perfusion liquid contained 1.5 g/l glucose. 1) L-isoprenaline (0.05 µM), a stimulating agent of the β-adrenergic receptors, provoked only a slight and brief stimulation of insulin secretion from the rat pancreas. At the same concentration, this substance provoked a considerable stimulation of the insulin secreting cell of the newborn dog pancreas and a still much more marked response from the uncinate process and the splenic portion of adult dog pancreas. The effects of salbutamol (0.09 µM), a stimulating agent of the β-adrenergic receptors, were comparable to those of isoprenaline. The effects of isoprenaline and salbutamol on these preparations were suppressed by propranolol. 2) As to the effects of L-adrenaline on these different preparations, the following results have been recorded: a) Adrenaline (0.011 µM) inhibited insulin secretion by rat pancreas; this effect was suppressed by phenoxybenzamine; it was not modified by propranolol. — b) Adrenaline (0.011 µM) exerted an inhibitory effect on newborn dog pancreas, but only temporarily. Phenoxybenzamine inversed the effects of adrenaline and allowed the appearance of stimulation of insulin secretion; propranolol accentuated the inhibitory effect of adrenaline. — c) On the uncinate process of the adult dog pancreas adrenaline (0.011 µM) provoked a biphasic effect: brief and strong rise of insulin secretion followed by slight inhibition. Phenoxybenzamine enhanced the adrenaline-induced stimulation and transformed the inhibition of the second phase into strong stimulation. Propranolol suppressed the stimulatory effect of adrenaline which then had only an immediate and strong inhibitory effect. Adrenaline at 0.055 µM concentration provoked an extremely brief hypersecretion peak which was less intense than that recorded when using the 5-times weaker concentration; this peak was followed by strong and lasting inhibition.
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References
Bacchus R. A., Meade L., Hutchinson J. S. M., London D. R.: The Control of Arginine-Induced Insulin Secretion in the Rat: the Role of Catecholamines — J. Endocr.58, 111, 1973.
Christensen N. J., Iversen J.: Release of Large Amounts of Noradrenaline from the Isolated Perfused Canine Pancreas during Glucose Deprivation — Diabetologia9, 396, 1973.
Cullum V. A., Farmer J. B., Jack D., Levy G. P.: Salbutamol: a New Selective β-Adrenoceptive Receptor Stimulant — Brit. J. Pharmacol.35, 141, 1969.
French J. W., Porte D. Jr., Baum D.: The Effect of Hypoxia and Epinephrine Infusion on Arginine Induced Insulin Release — Proc. Soc. exp. Biol. (N. Y.)144, 288, 1973.
Hales C. N., Randle P. J.: Immunoassay of Insulin with Insulin-Antibody Precipitate — Biochem. J.88, 137, 1963.
Loubatières A., Alric R., Mariani M. M., Chapal J.: Pancréas isolé et perfusé du rat. Fiche technique — J. Pharmacol. (Paris)3, 103, 1972.
Loubatières A., Mariani M. M.: Pancréas et catécholamines — J. Physiol. (Paris)56, 395, 1964.
Loubatières A., Mariani M. M.: Preuve pharmacologique directe que les récepteurs β-adrénergiques impliqués dans l’insulino-sécrétion sont de type β2 — C. R. Acad. Sci. (Paris)271, 1046, 1970.
Loubatières A., Mariani M. M., Chapal J.: Étude physiologique et pharmacologique de l’insulino-sécrétion sur le pancréas isolé et perfusé du rat. II. Effets des catécholamines et des substances bloquant les récepteurs adrénergiques — J. Pharmacol. (Paris)1, 227, 1970.
Loubatières A., Mariani M. M., Chapal J.: Insulino-sécrétion étudiée sur le pancréas isolé et perfusé du rat. II. Action des catécholamines et des substances bloquant les récepteurs adrénergiques — Diabetologia6, 533, 1970.
Loubatières A., Mariani M. M., Chapal J., Portal A.: Action frénatrice de faibles doses d’adrénaline et de noradrénaline sur l’insulino-sécrétion étudiée sur le pancréas isolé et perfusé du rat — C. R. Soc. Biol. (Paris)161, 2578, 1967.
Loubatières A., Mariani M. M., Ribes G., De Malbosc H., Chapal J.: Étude expérimentale d’un nouveau sulfamide hypoglycémiant particulièrement actif, le HB 419 ou glibenclamide. I. Action bêta-cytotrope et insulino-sécrétrice — Diabetologia5, 1, 1969.
Loubatières A., Mariani M. M., Savi L., Sorel G.: Étude pharmacologique du rôle des récepteurs β-adrénergiques dans l’insulino-sécrétion chez le chien — C. R. Soc. Biol. (Paris)164, 632, 1970.
Loubatières A., Mariani M. M., Sorel G., Savi L.: The Action of Beta-Adrenergic Blocking and Stimulating Agents on Insulin Secretion. Characterization of the Type of Beta Receptor — Diabetologia7, 127, 1971.
Loubatières-Mariani M. M., Chapal J., Ribes G., Loubatières A.: Variations suivant l’espèce animale de la réponse insulinique à l’activation des récepteurs bêta-adrénergiques — C. R. Soc. Biol. (Paris)168, 1033, 1974.
Loubatières-Mariani M. M., Chapal J., Ribes G., Loubatières A.: Différence dans l’intensité de la réponse insulinique à l’activation des récepteurs bêta-adrénergiques chez le chien et le rat — J. Pharmacol. (Paris)6, 105, 1975.
Mariani M. M., Chapal J., Loubatières A.: Isoprénaline, propranolol et insulino-sécrétion. Étude sur le pancréas isolé et perfusé du rat — C. R. Soc. Biol. (Paris)163, 2390, 1969.
Mariani M. M., Chapal J., Loubatières A.: Adrénaline, phénoxybenzamine et insulino-sécrétion. Étude sur le pancréas isolé et perfusé du rat — C. R. Soc. Biol. (Paris)163, 700, 1969.
Mariani M. M., Loubatières A., Sorel G., Savi L.: Insulino-sécrétion et récepteurs β-adrénergiques. Détermination du type de récepteur β impliqué — J. Pharmacol. (Paris)2, 473, 1971.
Nickerson M.: Adrenergic Receptors — Circulat. Res.32 (Suppl. 1), 53, 1973.
Porte D. Jr.: A Receptor Mechanism for the Inhibition of Insulin Release by Epinephrine in Man — J. clin. Invest.46, 86, 1967.
Porte D. Jr.: Beta Adrenergic Stimulation of Insulin Release in Man — Diabetes16, 150, 1967.
Porte D. Jr., Graber A. L., Kuzuya T., Williams R. H.: The Effect of Epinephrine on Immunoreactive Insulin Levels in Man — J. clin. Invest.45, 228, 1966.
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Loubatières-Mariani, MM., Chapal, J., Ribes, G. et al. Discrepancies in the response of the insulin secreting cells of the dog and rat to different adrenergic stimulating agents. Acta diabet. lat 14, 144–155 (1977). https://doi.org/10.1007/BF02581402
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DOI: https://doi.org/10.1007/BF02581402