Skip to main content
SpringerLink
Log in

Adoptive transfer of immunodiabetes in guinea pig

  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Summary

Inoculation of lymph node or spleen cells from diabetic guinea pigs producing precipitating anti-insulin antibodies produced signs of immunodiabetes in young guinea pigs with cyclophosphamide-suppressed immunological reactivity. Blood sugar increased on day 5 after the cell transfer and distinct B-cell degranulation in the islets of Langerhans was noted on day 7. No distinct insulitis occurred, but infiltrates rich in plasma cells were found in the exocrine pancreas and parapancreatic tissue. Similar infiltration and slight decrease of β-granules was observed in permanently normoglycemic recipients of normal lymphoid cells. In control animals treated with cyclophosphamide only no such a reaction occurred.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Armin J., Grant R. T., Wright P. H.: Acute Insulin Deficiency Provoked by Single Injection of AIS - J. Physiol. (Lond.)153, 131, 1960.

    CAS  Google Scholar 

  2. Caramia F., Munger B. L., Lacy P. E.: The Ultrastructural Basis for the Identification of Cell Types in the Pancreatic Islets. I. Guinea Pig - Z. Zellforsch.67, 533, 1965.

    Article  PubMed  CAS  Google Scholar 

  3. Falk G. A., Kies M. W., Alvord E. C. Jr.: Passive Transfer of Experimental Allergic Encephalomyelitis: Mechanism of Suppression - J. Immunol.103, 1248, 1969.

    PubMed  CAS  Google Scholar 

  4. Freytag G. H.: Immunopathologie des Diabetes mellitus - G. Fischer, Stuttgart, 1972.

    Google Scholar 

  5. Gregor W. H., Martin J. M., Williamson J. R., Lacy P. E., Kipnis D. M.: A Study of the Diabetic Syndrome Produced in Rats by Anti-Insulin Serum - Diabetes12, 73, 1963.

    PubMed  CAS  Google Scholar 

  6. Grodsky G. M., Feldman R., Toreson W. E., Lee J. C.: Diabetes Mellitus in Rabbits Immunized with Insulin - Diabetes15, 579, 1966.

    PubMed  CAS  Google Scholar 

  7. Janković B. D., Mitrović K., Popesković L., Milošević D.: Passive Transfer of Experimental Allergic Thyroiditis, Delayed Hypersensitivity to Thyroglobulin and Anti-Thyroglobulin Antibody Formation in Inbred Lewis Rats- Clin. exp. Immunol.5, 29, 1969.

    PubMed  Google Scholar 

  8. Kantor G. L., Dixon F. J.: Transfer of Experimental Allergic Orchitis with Peritoneal Exudate Cells - J. Immunol.108, 329, 1972.

    PubMed  CAS  Google Scholar 

  9. Korčáková L., Titlbach M., Lomský R.: Immunodiabetes in the Guinea Pig. 1st Part: Light Microscopy - Z. mikr.-anat. Forsch.85, 85, 1972.

    PubMed  Google Scholar 

  10. LeCompte R. M., Steinke J., Soeldner J. S., Renold A. E.: Changes in the Islets of Langerhans in Cows Injected with Heterologous and Homologous Insulin - Diabetes15, 586, 1966.

    PubMed  CAS  Google Scholar 

  11. Logothetopoulos J.: Electron Microscopy of the Pancreatic Islets Stimulated by Insulin Antibody - Canad. J. Physiol. Pharmacol.46, 407, 1968.

    CAS  Google Scholar 

  12. Logothetopoulos J., Bell E. G.: Histological and Autoradiographic Studies on the Islets of Mice Injected with Insulin Antibody - Diabetes15, 205, 1966.

    PubMed  CAS  Google Scholar 

  13. Moloney P. J., Coval M.: Antigenicity of Insulin: Diabetes Induced by Specific Andibodies - Biochem. J.59, 179, 1955.

    PubMed  CAS  Google Scholar 

  14. Nakamura R. M., Weigle W. O.: Passive Transfer of Experimental Autoimmune Thyroiditis from Donor Rabbits Injected with Soluble Thyroglobulin without Adjuvant - Int. Arch. Allergy32, 506, 1967.

    PubMed  CAS  Google Scholar 

  15. Paterson P. Y., Hanson M. A.: Cyclophosphamide Inhibition of Experimental Allergic Encephalomyelitis and Cellular Transfer of the Disease in Lewis Rats - J. Immunol.103, 1311, 1969.

    PubMed  CAS  Google Scholar 

  16. Petterson B.: The Two Types of Alpha Cells during the Development of Guinea Pig Pancreas - Z. Zellforsch.75, 371, 1966.

    Article  Google Scholar 

  17. Renold A. E., Gonet A. E., Vecchio D.: Immunopathology of the Endocrine Pancreas - In:Miescher P. A., Müller-Eberhard H. J. (Eds): Textbook of Immunopathology. Grune & Stratton, New York-London, 1969; p. 595.

    Google Scholar 

  18. Robinson B. H. B., Wright P. H.: Guinea Pig Anti-Insulin Serum- J. Physiol. (Lond.)155, 302, 1965.

    Google Scholar 

  19. Titlbach M., Korčáková L.: Immunodiabetes in the Guinea Pig. 2nd Part: Electron Microscopy - Z. mikr.-anat. Forsch.85, 199, 1972.

    PubMed  CAS  Google Scholar 

  20. Toreson W. E., Lee J. C., Grodsky G. M.: The Histopathology of Immune Diabetes in the Rabbit - Amer. J. Path.52, 1099, 1968.

    PubMed  CAS  Google Scholar 

  21. Tung K. S. K., Unanue E. R., Dixon F. J.: Pathogenesis of Experimental Allergic Orchitis. I. Transfer with Immune Lymph Node Cells - J. Immunol.106, 1453, 1971.

    PubMed  CAS  Google Scholar 

  22. Yokoo S., Aozi O., Okumura K., Fujh M., Yoshida H.: Study on the Formation and Release of Insulin with Special Reference to Electron Microscopic Observations - Arch. histol. jap.27, 296, 1966.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut Klinické a Experimentální Mediciny, Praha

    Lidka Korčáková, Milan Titlbach & Karel Nouza

  2. Československá Akademie Věd Laboratoř Pro Výzkum Ultrastruktury Buněk a Tkání Praha, Czechoslovakia

    Lidka Korčáková, Milan Titlbach & Karel Nouza

  3. Ústav Experimentální Biologie a Genetiky, Praha

    Lidka Korčáková, Milan Titlbach & Karel Nouza

Authors
  1. Lidka Korčáková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Milan Titlbach
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karel Nouza
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Korčáková, L., Titlbach, M. & Nouza, K. Adoptive transfer of immunodiabetes in guinea pig. Acta diabet. lat 11, 112–135 (1974). https://doi.org/10.1007/BF02581311

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02581311

Key-words

Search

Navigation