Summary
Pharmacodynamic characteristics of pentoxyfylline (BL 191) related to insulin secretion by the isolated perfused rat pancreas are studied.
The results obtained show that: 1) BL 191 (5 mM) is capable of stimulating insulin secretion, even in the presence of another stimulator; 2) BL 191 increases both phases of the secretion produced by constant arginine 20 mM/glucose 5 mM perfusion; 3) BL 191 significantly increases and turns biphasic the monophasic insulin secretion pattern produced by 1 µg/ml glibenclamide; 4) the effects mentioned in points 2) and 3) are inhibited if the phosphodiesterase activator imidazole (300 mg/100 ml) is present in the perfusion medium; 5) the phosphodiesterase inhibitor theophylline has the same effects as BL 191, except for its inability to stimulate insulin release in the absence of another stimulator; 6) somatostatin (100 ng/ml) significantly inhibits insulin secretion produced by arginine/glucose or glibenclamide, as well as by arginine, glucose plus theophylline or BL 191, and by glibenclamide plus theophylline or BL 191; in both cases the inhibitory effect of somatostatin is reduced by the presence of BL 191 or theophylline.
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References
Alberti K. G. M. M., Christensen S. E., Iversen J., Seyer-Hansen K., Christensen N. J., Hansen A. P., Lundbaek K., Ørskov H.: Inhibition of Insulin Secretion by Somatostatin — Lancet2, 1299, 1973.
Basabe J. C., Cresto J. C., Aparicio N., Schally A. V.: Studies on the Mode of Action of Somatostatin on Insulin Secretion — Endocrinology, 1977 (in press).
Basabe J. C., Lopez N. L., Viktora J. K., Wolff F. W.: Studies of Insulin Secretion in the Perfused Rat Pancreas. Effect of Diazoxide and A025 — Diabetes19, 271, 1970.
Basabe J. C., Lopez N. L., Viktora J. K., Wolff F. W.: Insulin Secretion Studied in the Perfused Rat Pancreas. II. Effect of Glucose, Glucagon, 3′, 5′ Adenosine Monophosphate, Theophylline, Imidazole and Phenoxybenzamine; their Interaction with Diazoxide — Diabetes20, 457, 1971.
Boksay I., Bollmann V.: The Effect of 3,7-Dimethyl-1(5-Oxohexyl)-Xanthine on the Adrenergic Receptors and on the Activity of Isoprenaline — Arch. int. Pharmacdoyn.194, 174, 1974.
Cerasi E., Luft R.: The Effect of an Adenosine-3′, 5′-Monophosphate Diesterase Inhibitor (Aminophylline) on the Insulin Response to Glucose Infusion in Prediabetic and Diabetic Subjects — Hormone metab. Res.1, 162, 1969.
Charles M. A., Fanska R., Schmid F. D., Grodsky G. M.: Adenosine 3′, 5′-Monophosphate in Pancreatic Islets: Glucose Induced Insulin Release — Science179, 569, 1973.
Cresto J. C., Dujovne I. L., Castellani P. I., Mitta E. A., De Majo S. F., Foglia V. G.: Insulin Radioimmunoassay by the Charcoal-Dextran Technique — Diabetologia8, 292, 1972.
Curry D., Bennett L. L., Grodsky G. M.: Dynamics of Insulin Secretion by the Perfused Rat Pancreas — Endocrinology83, 72, 1968.
Dollinger H. C., Raptis S., Grebe B., Goebell H., Müller R., Hinze H., Pfeiffer E. F.: The Influence of 1-(5-Oxohexyl-)3,7-Dimethylxantine (BL 191) on the Endocrine and Exocrine Pancreatic Function in Man during and following Secretion and Cholecystokinin-Pancreozymin Stimulation — Res. exp. Med.161, 327, 1973.
Efendić S., Luft R.: Studies on the Inhibitory Effect of Somatostatin on Glucose Induced Insulin Release in the Isolated Perfused Rat Pancreas — Acta endocr. (Kbh.)78, 510, 1975.
Fujimoto W. Y., Ensinck J. W.: Somatostatin Inhibition of Insulin and Glucagon Secretion in Rat Islet Culture: Reversal by Ionophore A 23187 — Endocrinology98, 259, 1976.
Gerich J. E., Lorenzi M., Schneider V., Forsham P. H.: Effect of Somatostatin on Plasma Glucose and Insulin Responses to Glucagon and Tolbutamide in Man — J. clin. Endocr.39, 1057, 1974.
Herbert V., Lan K., Gottlieb S., Bleicher S.: Coated Charcoal Radioimmunoassay of Insulin — J. clin. Endocr.25, 1375, 1965.
Malaisse W., Malaisse-Lagae F., King S.: Effect of Neutral Red and Imidazole upon Insulin Secretion — Diabetologia4, 370, 1968.
Malaisse W., Malaisse-Lagae F., Mayhew D.: A Possible Role for the Adenylcyclase System in Insulin Secretion — J. clin. Invest.46, 1724, 1967.
Montague W., Cock J. R.: The Role of Adenosine 3′, 5′-Cyclic Monophosphate in the Regulation of Insulin Release by Isolated Rat Islets of Langerhans — Biochem. J.122, 115, 1971.
Penhos J. C., Wu C., Basabe J. C., Lopez N. L., Wolff F.: A Rat Pancreas Small Gut Preparation for the Study of Intestinal Factor(s) and Insulin Release — Diabetes18, 733, 1969.
Porte D. Jr., Pupo A.: Insulin Release to Glucose: Evidence for a Two Pool System in Man — J. clin. Invest.48, 2309, 1969.
Sieradzki J., Schatz H., Nierle Ch., Pfeiffer E. F.: Somatostatin-Induced Inhibition of Insulin Secretion from Isolated Islets of Rat Pancreas in Presence of Glucagon — Hormone metab. Res.7, 284, 1975.
Turtle J., Kipnis D.: Stimulation of Insulin Secretion by Theophylline — Nature (Lond.)213, 727, 1967.
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Basabe, J.C., Udrisar, D.P., Knopf, C.F. et al. The influence of pentoxyfylline [1-(5-oxohexyl-) 3,7-dimethylxanthine] (BL 191) on the insulin secretion induced by glibenclamide and by arginine/glucose in the perfused pancreas. Acta diabet. lat 14, 263–272 (1977). https://doi.org/10.1007/BF02580975
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DOI: https://doi.org/10.1007/BF02580975