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Effects of sodium butyrate on the transfer of arachidonic acid to phosphatidylcholine in a clonal oligodendrocyte cell line (CB-II)

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Lipids

An Erratum to this article was published on 01 December 1994

Abstract

The effect of sodium butyrate on membrane phospholipid metabolism in a neonate rat cerebellum derived clonal oligodendrocyte cell line (CB-II) was investigated. Sodium butyrate is an agent known to induce cell differentiation and morphological transformations. A comparison of thein vivo phospholipid labeling patterns obtained by incubating CB-II cells with [3H]choline, [14C]myristic acid or [3H]arachidonic acid indicated that butyrate altered the route of acylation-deacylation in phosphatidylcholine (PC) biosynthesis. Using anin vitro incubation system containing homogenates of CB-II cells, the largest proportion of radioactivity was found in PC, and addition of sodium butyrate resulted in a further increase in the transfer of arachidonic acid to PC, but not to phosphatidylinositol. Similar results were obtained when thisin vitro acylation activity was tested using homogenates from sodium butyrate pretreated cells. The butyrate effect was observed regardless of whether or not exogenous lysophosphatidylcholine (LPC) was added to the incubation system. Addition of butyrate did not result in a change in the activity of LPC:acyl-CoA (coenzyme A) acyltransferase (EC 2.3.1.23) in CB-II cells upon incubating cell homogenates with [1-14C]arachidonoyl-CoA and LPC. However, when cell homogenates were incubated with [3H]arachidonic acid in the presence of 2.5–10 mM sodium butyrate, arachidonoyl-CoA synthesis was stimulated. A time course study demonstrated that significant stimulation occurred after three minutes. Taken together, the results suggest that in CB-II cells, sodium butyrate stimulates the transfer of arachidonic acid into PC and that this effect is at least partially due to a stimulation of arachidonoyl-CoA ligase (EC 6.2.1.3).

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Abbreviations

ATP:

adenosine triphosphate

BSA:

bovine serum albumin

CB-II:

neonate rat cerebellum-derived clonal oligodendrocyte

CoA:

coenzyme A

FBS:

fetal bovine serum

HPTLC:

high-performance thin-layer chromatography

LPC:

lysophosphatidylcholine

LPI:

lysophosphatidylinositol

LPE:

lysophosphatidylethanolamine

LPS:

lysophosphatidylserine

PBG:

glucose supplemented phosphate buffer

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PEpl:

ethanolamine plasmalogen

PI:

phosphatidylinositol

PS:

phosphatidylserine

TG:

triacylglycerol(s)

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Authors and Affiliations

  1. Department of Anatomy, National Yang Ming Medical College, Taipei, Taiwan, Republic of China

    Kuo-Chi Chen

  2. Institute of Neuroscience, National Yang Ming Medical College, No. 155, Section 2, Li-Non Street, Shih Pai, Taipei, Taiwan, Republic of China

    Synthia H. Sun & Yue-Wen Chen

Authors
  1. Synthia H. Sun
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  2. Kuo-Chi Chen
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  3. Yue-Wen Chen
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Additional information

A correction to this article is available at http://dx.doi.org/10.1007/BF02536263

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Sun, S.H., Chen, KC. & Chen, YW. Effects of sodium butyrate on the transfer of arachidonic acid to phosphatidylcholine in a clonal oligodendrocyte cell line (CB-II). Lipids 29, 467–474 (1994). https://doi.org/10.1007/BF02578243

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  • DOI: https://doi.org/10.1007/BF02578243

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