Skip to main content
SpringerLink
Log in

Lipid peroxidation and antioxidant activities in regenerating liver after partial hepatectomy in splenectomized rats

  • Original Papers
  • Published:
Research in Experimental Medicine

Abstract

Lipid peroxidation, antioxidant activities, and total fatty acids were investigated in the regenerating liver after partial hepatectomy in splenectomized male Wistar rats. The lipoperoxide levels were significantly lower in the splenectomized rats than in the non-splenectomized rats. The hepatic superoxide dismutase (SOD) activity was significantly higher in the splenectomized rats than in the controls, whereas the α-tocopherol content, and the activities of glutathione (GSH) peroxidase and catalase did not differ so widely between splenectomized and nonsplenectomized rats. In both groups, the fatty acid composition of the total lipids was not so different in the early stages of hepatic regeneration after partial hepatectomy, but it was noted that in the later stages of hepatic regeneration, polyunsaturated fatty acids tended to increase in the splenectomized rats. These results suggest that splenectomy attenuates lipid peroxidation, and tends to increase in polyunsaturated fatty acids in the remnant liver after partial hepatectomy, which may be attributable to high SOD activity, the maintenance of α-tocopherol, and the remaining activities of GSH peroxidase and catalase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GSH:

Glutathione

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

References

  1. Abe K, Ohmae M, Katsui G (1976) Rapid and micro-method for the determination of tocopherols in liver. Vitamins (Japan) 50:453–457

    CAS  Google Scholar 

  2. Billiar TB, West MA, Hyland BJ, Simmons RL (1988) Splenectomy alters Kupffer cell response to endotoxin. Arch Surg 123:327–332

    PubMed  CAS  Google Scholar 

  3. Cheeseman KH, Collins M, Maddix S, Milia A, Proudfoot K, Slater TF, Burton GW, Webb A, Ingold KU (1986) Lipid peroxidation in regenerating rat liver. FEBS Lett 209: 191–196

    Article  PubMed  CAS  Google Scholar 

  4. Cockerill MJ, Player TJ, Horton AA (1982) Studies on lipid peroxidation in regenerating rat liver. Biochim Biophys Acta 750:208–213

    Google Scholar 

  5. Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34:30–38

    Article  PubMed  CAS  Google Scholar 

  6. Fukunishi K (1991) Effects of superoxide dismutase on liver regeneration in rats. Nippon Geka Gakkai Zasshi 192:339–345

    Google Scholar 

  7. Gavino VC, Dillard CJ, Tappel AL (1985) Effect of dietary vitamin E and Santoquin on regenerating rat liver. Life Sci 36:1771–1777

    Article  PubMed  CAS  Google Scholar 

  8. Hafeman DG, Sunde RA, Hoekstra WG (1974) Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr 104:580–587

    PubMed  CAS  Google Scholar 

  9. Hara A, Taketomi T (1990) Characterization and change of phospholipids in the aorta of Watanabe hereditable hyperlipidemic rabbit. Jpn J Exp Med 60:311–318

    PubMed  CAS  Google Scholar 

  10. Higgins GM, Anderson RM (1931) Experimental pathology of the liver. Arch Pathol 12:186–202

    Google Scholar 

  11. Ishihara H, Tamiya K, Kuriki H, Yoshida S, Kojima K (1991) Growth-associated changes in fatty acid compositions of nuclear phospholipids of liver cells. Biochim Biophys Acta 1084:53–59

    PubMed  CAS  Google Scholar 

  12. Ito Y, Hiraishi H, Razandi M, Terano A, Hirada T, Ivey KJ (1992) Role of cellular superoxide dismutase against reactive oxygen metabolite-induced cell damage in cultured rat hepatocytes. Hepatology 16:247–254

    Article  PubMed  CAS  Google Scholar 

  13. Kubo S, Matsui I, Otani S, Morisawa S, Kinoshita H, Sakai K (1985) Effect of splenectomy on liver regeneration after partial hepatectomy. On the metabolism of polyamine. Jpn J Gastroenterol 82:100–107

    CAS  Google Scholar 

  14. Kuo PC, Schroeder RA (1995) The emerging multifaceted roles of nitric oxide. Ann Surg 221:220–235

    Article  PubMed  CAS  Google Scholar 

  15. Kurokawa T, Nonami T, Sugiyama S, Ozawa T, Takagi H (1991) Effects of long-acting superoxide dismutase on liver metabolism after major hepatic resection in rats with cirrhosis. Eur Surg Res 23:65–72

    PubMed  CAS  Google Scholar 

  16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  17. Maheshwari HB, Kumar S (1979) Effect of splenectomy on liver restoration after partial hepatectomy in albino rats. Indian J Exp Biol 17:911–914

    PubMed  CAS  Google Scholar 

  18. Nadkarni GD, Mitra AG, Deshpande UR, Pahuja DN (1991) Liver antioxidant defense and lipid peroxidation in vitamin D-deprived rats. Indian J Biochem Biophys 28:224–225

    PubMed  CAS  Google Scholar 

  19. Nakagawa M, Hiramatsu Y, Fukubayashi H, Mitsuyoshi K, Yamamura M, Hioki K, Yamamoto M (1991) Comparison of effects of long-chain and medium-chain triglyceride emulsions during hepatic regeneration in rats. Nutrition 7:23–27

    PubMed  CAS  Google Scholar 

  20. Ohira M, Umeyama K, Taniura M, Yamashita T, Morisawa S (1987) An experimental study of a splenic inhibitory factor influencing hepatic regeneration. Surg Gynecol Obstet 164:438–444

    PubMed  CAS  Google Scholar 

  21. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  22. Okuyama Y, Shinzawa H, Ukai K, Ono K, Togashi H, Wakabayashi H, Yamada N, Nakamura T, Takahashi T, Ishikawa M (1991) Changes of Cu, Zn-SOD and LPO in the regenerating liver after partial hepatectomy. Effect of coenzyme Q10 administration. Jpn J Gastroenterol 88:1208–1215

    CAS  Google Scholar 

  23. Oyanagui Y (1984) Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142:290–296

    Article  PubMed  CAS  Google Scholar 

  24. Poli G, Albano E, Dianzani M (1987) The role of lipid peroxidation in liver damage. Chem Phys Lipids 45:117–142

    Article  PubMed  CAS  Google Scholar 

  25. Singh S, Shackleton G, Ah-Sing E, Chakraborty J, Bailey ME (1992) Antioxidant defenses in the bile duct-ligated rat. Gastroenterology 103:1625–1629

    PubMed  CAS  Google Scholar 

  26. Slater TF, Cheeseman KH, Benedetto C, Collins M, Emery S, Maddix SP, Nodes JT, Proudfoot K, Burton GW, Ingold KU (1990) Studies on the hyperplasia (‘regeneration’) of the rat liver following partial hepatectomy: changes in lipid peroxidation and general biochemical aspects. Biochem J 265:51–59

    PubMed  CAS  Google Scholar 

  27. Spitzer JA (1994) Cytokine stimulation of nitric oxide formation and differential regulation in hepatocytes and nonparenchymal cells of endotoxemic rats. Hepatology 19:217–228

    Article  PubMed  CAS  Google Scholar 

  28. Taniura K (1986) Effect of splenectomy on hepatic regeneration in rats. Nippon Geka Gakkai Zasshi 87:423–434

    PubMed  CAS  Google Scholar 

  29. Tsai JL, King KL, Chang CC, Wei YH (1992) Changes of mitochondrial respiratory functions and superoxide dismutase activity during liver regeneration. Biochem Int 28:205–217

    PubMed  CAS  Google Scholar 

  30. Ueda K, Yoshioka T, Takehara Y, Abe K (1983) Lipoperoxides, vitamin E, and activities of superoxide dismutase, glutathione peroxidase, and catalase in regenerating rat liver. Biochem Int 7:663–669

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Surgery, Shinshu University School of Medicine, 3-1-1, Asahi, 390, Matsumoto, Nagano-ken, Japan

    Toshio Imai, Keiji Nishimaki, Tomoyuki Shiga, Seiji Kawasaki & Masatoshi Makuuchi

Authors
  1. Toshio Imai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keiji Nishimaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tomoyuki Shiga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Seiji Kawasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masatoshi Makuuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Imai, T., Nishimaki, K., Shiga, T. et al. Lipid peroxidation and antioxidant activities in regenerating liver after partial hepatectomy in splenectomized rats. Res. Exp. Med. 196, 1–7 (1996). https://doi.org/10.1007/BF02576823

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02576823

Key words

Search

Navigation