Abstract
Twelve pigs underwent orthotopic liver transplantation. The mean endothelin-1 (ET-1) levels in the serum samples of the recipient animals 1 h after reperfusion of the graft (6.2±1.5 pg/ml) was significantly higher (P<0.05) than in pretransplantation samples (3.2±0.6 pg/ml). Serum blood urea nitrogen (BUN) 24 h after transplantation was 13.8±5.9 mg/dl, which was significantly higher than before transplantation (6.4±2.2 mg/dl). There was a positive correlation between the serum BUn and ET-1 (r=0.62,P<0.05). An in vitro isometric tension study was performed for the contractility response rate of the intact renal artery in the bath chamber containing the serum of the corresponding recipient animals. The mean contractility response rates were higher with the serum obtained after reperfusion of the graft (66.9±32.4%) than with those obtained before transplantation (18.3±9.2%) when compared to a standard contractility rate of 100% with 40 mM KCl. Moreover, these contractility response rates were significantly reduced (32.8±21.0%) with the addition of the ET-1 receptor antagonist FR139 317. The results of the present study demonstrated that the liver transplantation was associated with elevation of ET-1 in the serum of the recipient animals. It was considered that ET-1 in the serum caused a direct vasoconstriction of the renal artery in vitro. This may help to explain the renal dysfunction that is often seen in the recipients of clinical liver transplantation.
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Shaw BW Jr, Wood RP (1989) Improved results with retransplantation of the liver. Transplant Proc 21:2407–2408
Clavien PA, Harvey RC, Strasberg SM (1992) Preservation and reperfusion injuries in liver allograft. Transplantation 53:957–978
Popolawski SC, Gonwa, Goldstein RM, Klintmalm GB (1989) Renal dysfunction following orthotopic liver transplantation. Clin Transplant 3:94–100
Yokoyama I, Todo S, Miyata T, Selby R, Tzakis AG, Starzl TE (1989) Endotoxemia and human liver transplantation. Transplant Proc 21:3833–3841
Post S, Georig M, Otto G, Manner M, Senninger N, Kommerell B, Herfarth C (1990) Prostanoid release in experimental liver transplantation. Transplantation 49:490–494
Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1987) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415
Lerman A, Click RL, Narr BJ, Wiesner RH, Krom RAF, Textor SC, Burnet JC Jr (1991) Elevation of plasma endothelin associated with systemic hypertension in humans following orthotopic liver transplantation. Transplantation 51:646–650
Stansby G, Fuller B, Jeremy J, Cheetham K, Rolles K (1993) Endothelin release—A facet of reperfusion injury in clinical liver transplantation? Transplantation 56:239–240
Textor SC, Wilson DJ, Lerman A, Romero JC, Burnett JC Jr, Wiesner R, Dickson ER, Krom RAF (1992) Renal hemodynamics, urinary eicosanoids, and endothelin after liver transplantation. Transplantation 54:74–80
Starzl TE, Iwatsuki S, Esquivel CO, Todo S, Kam I, Lynch S, Gordon RD, Shaw BS Jr (1985) Refinements in surgical technique of liver transplantation. Semin Liver Dis 5:349–356
Suzuki N, Matsumoto H, Kitada C, Masaki T, Fujino M (1989) A sensitive sandwich-enzyme immunoassay for human endothelin. J Immunol Methods 118:245–250
Hayakawa A, Kawanishi M, Ishikawa N, Shimada Y, Goto K (1993) Different mode of action of endothelin 1 in parietal and visceral veins. Eur J Pharmacol 239:219–222
Battistini B, D'Orléans-Juste P, Sirois P (1993) Biology of disease. Endothelins: Circulating plasma levels and presence in other biologic fluids. Lab Invest 68:600–628
Firth JD, Ratcliffe PJ, Raine AEG, Ledingham JGG (1988) Endothelin: An important factor in acute renal failure? Lancet 19:1178–1179
Takabatake T, Ise T, Ohta K, Kobayashi K (1991) Endothelin effects on renal function and tubuloglomerular feedback. Kidney Int 39 [Suppl 32]:S122-S124
Kon V, Yoshioka T, Fogo A, Ichikawa I (1989) Glomerular actions of endothelin in vivo. J Clin Invest 83:1762–1767
Simonson MS (1993) Endothelins: Multifunctional renal peptides. Physiol Rev 73:375–411
Shibata R, Yanagisawa M, Miyauchi T, Ishii Y, Kimura S, Uchiyama Y, Masaki T, Goto K (1989) Elimination of intravenously injected endothelin-1 from the circulation of the rat. J Cardiovasc Pharmacol 13 [Suppl 5]:S98-S101
Goto M, Takei Y, Kawano S, Nagano K, Tsuji S, Masuda E, Mishimura Y, Okumura S, Kashiwagi T, Fusamoto H, Kamada T (1994) Endothelin-1 is involved in the pathogenesis of ischemia/reperfusion liver injury by hepatic microcirculatory disturbances. Hepatology 19:675–681
Shibata Y, Suzuki N, Shino A, Matsumoto H, Terashita Z, Kondo K, Nishikawa K (1990) Pathophysiological role of endothelin in acute renal failure. Life Sci 46:1611–1618
Tsakiris DA, Leuscher TF, Loeffler BM, Marbet GA, Speck B, Gratwohl A (1994) Endothelin in the early phase of bone marrow transplantation. Transplantation 57:300–301
Sogabe K, Nirei H, Shoubo M, Nomoto A, Ao S, Notu Y, Ono T (1993) Pharmacological profile of FR139 317, a novel, potent endothelin ETA receptor antagonist. J Pharmacol Exp Ther 264:1040–1046
Kanse SM, Takahashi K, Warren FB, Ghatei M, Bloom SR (1991) Glucocorticoids induce endothelin release from vascular smooth muscle cells but not endothelial cells. Eur J Pharmacol 199:99–101
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Yokoyama, I., Hayakawa, A., Kobayashi, T. et al. Endothelin-1 and in vitro evidence of renal artery vasoconstriction after liver transplantation. Res. Exp. Med. 195, 381–387 (1995). https://doi.org/10.1007/BF02576809
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DOI: https://doi.org/10.1007/BF02576809