Abstract
Damage to the hepatic microcirculation plays an important part in the preservation-related loss of graft viability. In a study on the isolated, hypothermically preserved rat liver perfused continuously with Euro-Collins and University of Wisconsin solution (gluconate substituted for lactobionate) at different flow rates we determined changes in diameter of central veins and pericentral sinusoids and related them to weight changes. While post-perfusion liver weight increased with Euro-Collins and decreased with University of Wisconsin solution, weight changes did not vary significantly with different flow rates. Changes in sinusoidal diameter depended on the type of solution as well as on the flow rate. Cell swelling under Euro-Collins perfusion leads to compression of pericentral sinusoids, which varies in degree with different flow rates. Perfusion with University of Wisconsin solution under near-normal flow, due to slight shrinking of parenchymal cells, keeps the sinusoidal lumina wide open. We conclude that there is a causal relationship between perfusion-dependent cell swelling and reduction in sectional area of the hepatic microvasculature. Continuous hypothermic perfusion with University of Wisconsin solution at physiologic flow rates excellently preserves the size and trabecular architecture of hepatocytes and thereby the sinusoidal lumina within the hepatic lobules.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Belzer FO, Southard JH (1988) Principles of solid-organ preservation by cold storage. Transplantation 45: 673–676
Birnie JH, Grayson J (1952) Observations on temperature distribution and liver blood flow in the rat. J Physiol (Lond) 116: 189–201
Blumhardt G, Lemmens P, Topalidis T (1993) Increased flow rate of preservation solution in the hepatic artery during organ preservation can improve post-ischemic liver function. Transplant Proc 25: 2540–2541
Caldwell-Kenkel JC, Currin RT, Tanaka Y, Thurman RG, Lemasters JJ (1991) PatKupffer cell activation and endothelial cell damage after storage of rat livers: effects of reperfusion. Hepatology 13: 83–95
Chazouilleres O, Ballet F, Chretien Y, Marteau P, Rey C, Maillard D, Poupon R (1989) Protective effects of vasodilators on liver function after long hypothermic preservation: a study in the isolated perfused rat liver. Hepatology 9: 824–829
Clavien PA, Harvey PR, Strasberg SM (1992) Preservation and reperfusion injuries in liver allografts. Transplantation 53: 957–978
Greengard O, Federman M, Knox WE (1972) Cytomorphometry of developing rat liver and its application to enzymic differentiation. J Cell Biol 52: 261–272
Haba T, Hayashi S, Hachisuka T, Ootsuka S, Tanaka Y, Satou E, Takagi H (1992) Microvacular changes of the liver preserved in UW solution. Cryobiology 29: 310–322
Hernandez LA, Grisham MB, Twohig B, Arfors K, Harlan JM, Granger DN (1987) Role of neutrophils in ischemia-reperfusion-induced microvascular injury. Am J Physiol 253: H699-H703
Jamieson NV (1989) A new solution for liver preservation. Br J Surg 76: 107–108
Koizumi M, Ohkohchi N, Katoh H, Koyamada N, Fujimori K, Sakurada M (1989) Presevation and reflow damage in liver transplantation in the pig. Transplant Proc 21: 1323–1326
Marni A, Ferrero ME (1988) A four-technique comparative study of orthotopic liver transplantation in the rat. Am J Surg 156: 209–213
Marteau P, Ballet F, Chretien Y (1988) Effect of vasodilators on hepatic microcirculation: a study of the inhibition of norepinephrine-induced vasoconstriction in the isolated perfused rat liver. Hepatology 8: 228–231
Marzi I, Walcher F, Bühren V (1992) The influence of preservation solutions on the hepatic microcirculation after liver transplantation. In: Kirste G (ed) Custodiol Symposium. Innovations-Verlags-Gesellschaft, Seeheim, p 73
McKeown CMB, Edwards V, Phillips MJ, Harvey PR, Petrunka CN, Strasberg SM (1988) Sinusoidal lining cell damage: the critical injury in cold preservation of liver allografts in the rat. Transplantation 46: 178–191
Momii S, Koga A (1990) Time-related morphological changes in cold-stored rat livers. Transplantation 50: 745–750
Pienaar B, Lindell S, Gulik T van, Southard JH, Belzer FO (1990) Seventy-two-hour preservation of the canine liver by machine perfusion. Transplantation 49: 258–260
Rossaro L, Murase N, Caldwell C, Farghali H, Casavilla A, Starzl TE, Ho C, Thiel D van (1992) Phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. J Lab Clin Med 120: 559–568
Sundberg R, Arrajab A, Ahren B, Bengmark S (1991) The functional effects of suppression of hypothermia-induced cell swelling in liver preservation by cold storage. Cryobiology 28: 150–158
Takei Y, Marzi I, Gores GJ, Currin RT, Lemasters JJ, Thurman RG (1990) Videomicroscopy of transplanted rat livers. In: Herman B, Jacobson K (eds) Optical microscopy for biology. Liss, New York, p 487
Tamaki T, Kamada N, Pegg DE (1986) Hypothermic preservation of the rat liver assessed by orthotopic transplantation. A comparison of flush solutions. Transplantation 41: 396–397
Thurman RG, Bunzendahl H, Lemasters JJ (1993) Role of sinusoidal lining cells in hepatic reperfusion injury following cold storage and transplantation. Semin Liver Dis 13: 93–100
Van Gulik T, Lindell S, Boudjema K, Pienaar B, Southard JH, Belzer FO (1990) Combined cold storage and perfusion preservation of the canine liver. Transplant Proc 22: 520–522
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kasperk, R., Füzeti, L., Müller, M. et al. Morphometric study of centrilobular vessels in the rat liver after continuous hypothermic perfusion with Euro-Collins and University of Wisconsin solution. Res. Exp. Med. 194, 383–390 (1994). https://doi.org/10.1007/BF02576401
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02576401