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Dephosphorylation of nuclear non-histone proteins in submandibular glands of rats treated with isoproterenal

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Research in Experimental Medicine

Abstract

Protein phosphatase that removed [32P] phosphate from non-histone proteins, i.e., phenol-soluble acidic proteins, more rapidly and strongly than from histone proteins was present in nuclei of rat submandibular glands, but was not associated with chromatin. Cyclic AMP (10−4–10−2 mM) stimulated the dephosphorylation of non-histone proteins, but not that of histone proteins. After a single injection of isoproterenol (IPR), the dephosphorylation of non-histone proteins in rat submandibular gland nuclei increased within 15 min, reached a maximum in 30 min and returned to normal control levels within 4 h. The stimulation of dephosphorylation of non-histone proteins induced by IPR was not observed after prior treatment of the animals with dichloroisoproterenol. The dephosphorylation of histone proteins was not affected by the injection of IPR. Stimulation of β-adrenoceptors with IPR in rat submandibular glands resulted in increase in cyclic AMP and decrease in RNA synthesis in the tissues in the first few hours after the injection. This decrease in RNA synthesis was temporary and was preceded by the increase in cyclic AMP level and in the dephosphorylation of non-histone acidic proteins in the tissues. These results suggest that protein phosphatase in nuclei plays an important part in the events ontrolling RNA synthesis by regulating the state of phosphorylation of non-histone acidic proteins. In addition, the phosphatase may be regulated by a function of the cytoplasmic membranes.

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References

  1. Teng CS, Teng CT, Allfrey VG (1971) Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid and stimulatory effects on transcription. J Biol Chem 246:3597–3609

    PubMed  CAS  Google Scholar 

  2. Stein GS, Spelsberg TC, Kleinsmith LJ (1974) Nonhistone chromosomal proteins and gene regulation. Nonhistone chromosomal proteins may participate in the specific regulation of gene transcription in eukaryotes. Science 183:817–824

    Article  PubMed  CAS  Google Scholar 

  3. Ishikawa Y, Amano I, Chen C, Ishida H (1992) Diurnal variation of amylase secretion is coupled with alterations of β-adrenoceptors in the rat parotid gland. Res Exp Med 192:231–240

    CAS  Google Scholar 

  4. Rasmussen H (1970) Cell communication, calcium ion and cyclic nucleotide monophosphate. Science 170:404–412

    Article  PubMed  CAS  Google Scholar 

  5. Albano J, Bhoola KD, Heap PF, Lemon MJC (1976) Stimulus-secretion coupling. Role of cyclic AMP, cyclic GMP and calcium in mediating enzyme (kallikrein) secretion in the submandibular gland. J Physiol (Lond) 268:631–658

    Google Scholar 

  6. Hata F, Ishida H, Kagawa K, Kondo E, Kondo S, Noguchi Y (1983) β-adrenoceptor alterations coupled with secretory response in rat parotid tissue. J Physiol (Lond) 341:185–196

    CAS  Google Scholar 

  7. Baserga R (1966) Inhibition of stimulation of DNA synthesis by isoproterenol in submandibular glands of mice. Life Sci 5:2033–2039

    Article  CAS  Google Scholar 

  8. Baserga R, Hefler S (1967) Stimulation of DNA synthesis by isoproterenol and its inhibition by actinomysin D. Exp Cell Res 46:571–580

    Article  PubMed  CAS  Google Scholar 

  9. Barka T (1966) Stimulation of RNA synthesis in salivary gland by isoproterenol. Exp Cell Res 41:573–579

    Article  PubMed  CAS  Google Scholar 

  10. Barka T (1968) Stimulation of protein and ribonucleic acid synthesis in rat submaxillary gland by isoproterenol. Lab Invest 18:38–41

    PubMed  CAS  Google Scholar 

  11. Stein G, Baserga R (1970) The synthesis of acidic nuclear proteins in the prereplicative phase of the isoproterenol-stimulated salivary gland. J Biol Chem 245:6097–6105

    PubMed  CAS  Google Scholar 

  12. Ishida H, Ahmed K (1973) Studies on phosphoproteins of submandibular gland nuclei isolated from isoproterenol-treated rats. Exp Cell Res 78:31–40

    Article  PubMed  CAS  Google Scholar 

  13. Ishikawa Y, Chen C, Amano I, Koda N, Ishida H (1993) Age-dependent changes in the phosphorylation of nuclear proteins of submandibular glands in isoproterenol-treated rats. Mech Ageing Dev 70:127–137

    Article  PubMed  CAS  Google Scholar 

  14. Kleinsmith LJ, Stein J, Stein G (1976) Dephosphorylation of non-histone proteins specificaly alters the pattern of gene transcription in reconstituted chromatin. Proc Natl Acad Sci USA 73:1174–1178

    Article  PubMed  CAS  Google Scholar 

  15. Szeszak F, Pinna LA (1980) Protein phosphatase from rat liver nuclei. Mol Cell Biochem 32:13–20

    Article  PubMed  CAS  Google Scholar 

  16. Sahasrabuddha CG, Adlakha RC, Rao PN (1984) Phosphorylation of non-histone proteins associated with mitosis in hela cells. Exp Cell Res 153:439–450

    Article  Google Scholar 

  17. Abell CW, Monahan TM (1973) The role of adenosine 3′, 5′-cyclic monopphosphate in the regulation of mammalian cell division. J Cell Biol 59:549–558

    Article  PubMed  CAS  Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randoll RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:267–275

    Google Scholar 

  19. Ishida H, Ahmed K (1974) Studies on chromatin-associated protein phosphokinase of submandibular gland from isoproterenol-treated rats. Exp Cell Res 84:127–136

    Article  PubMed  CAS  Google Scholar 

  20. Maeno H, Greengard PJ (1971) Phosphoprotein phosphatase from rat cerebral cortex. Subcellular distribution and characterization. J Biol Chem 247:3269–3277

    Google Scholar 

  21. Stein GS, Farber J (1972) Role of non-histone chromosomal proteins in the restriction of mitotic chromatin template activity. Proc Natl Acad Sci USA 69:2918–2921

    Article  PubMed  CAS  Google Scholar 

  22. Schneider WC (1945) Phosphorous compounds in animal tissue. 1. Extraction and estimation of deoxypentose nucleic acid and of pentose nucleic acid. J Biol Chem 161:293–303

    CAS  Google Scholar 

  23. Otten J, Johnson GS, Pastan I (1971) Cyclic AMP levels in fibroblasts: relationship to growth rate and contact inhibition of growth Biochem Biophys Res Commun 44:1192–1198

    Article  PubMed  CAS  Google Scholar 

  24. Sheppard JR, Prescott DM (1972) Cyclic AMP level in synchronized mammalian cells. Exp Cell Res 75:293–296

    Article  PubMed  CAS  Google Scholar 

  25. Abell CW, Kamp CW, Johnson LD (1970) Effects of phytohemagglutinin and isoproterenol on DNA synthesis in lymphocytes from normal donors and patients with chronic lymphocytic leukemia. Cancer Res 30:717–723

    PubMed  CAS  Google Scholar 

  26. Johnson LD, Abell CW (1970) The effects of isoproterenol and cyclic adenosie 3′, 5′-phosphate on phytohemagglutinin-stimulated DNA synthesis in lymphocytes obtained from patients with chronic lymphocytic leukemia. Cancer Res 30:2718–2723

    PubMed  CAS  Google Scholar 

  27. Sea M, Kleinsmith LJ (1973) Template-specific stimulation of RNA synthesis by phosphorylated nonhistone chromatin proteins. Biochem Biophys Res Commun 50:473–477

    Article  Google Scholar 

  28. Kish VM, Kleinsmith LJ (1974) Nuclear protein kinases. Evidence for their heterogeneity, tissue specificity and differential responses to cyclic adenosine 3′, 5′-monophosphate. J Biol Chem 249:750–757

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, Tokushima University School of Dentistry, 3-Kuramoto-cho, 770, Tokushima City, Tokushima, Japan

    Ichiro Amano, Yasuko Ishikawa & Hajime Ishida

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  1. Ichiro Amano
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  2. Yasuko Ishikawa
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  3. Hajime Ishida
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Amano, I., Ishikawa, Y. & Ishida, H. Dephosphorylation of nuclear non-histone proteins in submandibular glands of rats treated with isoproterenal. Res. Exp. Med. 194, 185–196 (1994). https://doi.org/10.1007/BF02576379

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  • DOI: https://doi.org/10.1007/BF02576379

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