Summary
The preventive effect of β-alanyl-L-histidinato zinc (AHZ) on osteopenia was investigated in rats treated with hydrocortisone. Rats received hydrocortisone (75 mg/kg body weight per day) s.c. for 30 days. The steroid treatment caused a significant increase in serum alkaline phosphatase activity and parathyroid hormone (PTH-c) level, while serum calcium inorganic phosphorus, and zinc concentrations were not significantly altered. The femoral-diaphyseal alkaline phosphatase activity, deoxyribonucleic acid (DNA), and calcium contents were significantly decreased by the treatment of steroid, although the bone zinc content was not appreciably altered. When AHZ (10, 30, and 100 mg/kg per day) was administered p. o. for 30 days to rats giving the steroid, the dose of AHZ (30 and 100 mg/kg) completely prevented the increases in serum alkaline phosphatase activity and PTH-c level and the decreases in femoral-diaphyseal alkaline phosphatase activity, DNA, and calcium contents caused by the steroid treatment. The dose of AHZ (10, 30, and 100 mg/kg) significantly increased zinc content in the femoral diaphysis. Present results indicate that the dose of AHZ can prevent the disorder of bone metabolism caused by hydrocortisone treatment AHZ may have a therapeutic role in the steroid-induced osteopenia.
Similar content being viewed by others
References
Burch RE, Hahn JK, Sullivan F (1975) Newer aspects of the roles of zinc, manganese and copper in human nutrition. Clin Chem 21:501–520
Caniggia A, Nuti R, Lore F, Vattimo A (1981) Pathophysiology of the adverse effects of glucoactive corticosteroids on calcium metabolism in man. J Steroid Biochem 15:153–161
Ceriotti G (1955) Determination of nucleic acids in animal tissues. J Biol Chem 214:39–77
Flanagan B, Nichols G Jr (1962) Metabolic studies of bone in vitro: IV. Collagen biosynthesis by surviving bone fragments in vitro. J Biol Chem 237:3686–3692
Hshieh HS, Navia JM (1980) Zinc deficiency and bone formation guinea pig alveolar implants. J Nutr 110:1581–1588
Lowry OH, Rosebrough NH, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–273
Oner G, Bhagumick B, Bala RM (1984) Effect of zinc deficiency on serum somatomedin levels and skeletal growth in young rats. Endocrinology 114:1860–1863
Pock WA (1984) The effects of glucocorticoids on bone cell metabolism and function. Adv Exp Med Biol 171:111–119
Suzuki Y, Ichikawa Y, Saito E, Homma M (1983) Importance of increased urinary calcium excretion in the development of secondary hyperparathyroidism of patients under glucocorticoid therapy. Metabolism 32:151–156
Walter K, Schutt C (1965) Acid and alkaline phosphatase in serum. In: Bergmeyer HU (ed) Methods of enzymatic analysis (vols 1, 2). Academic Press, New York, pp 865–860
Yamaguchi M, Yamaguchi R (1986) Action of zinc on bone metabolism in rats: increases in alkaline phosphatase activity and DNA content. Biochem Pharmacol 35:773–777
Yamaguchi M, Ozaki K, (1990) A new zinc compound β-alanyl-L-histidinato zinc, stimulates bone growth in weanling rats. Res Exp Med 190:105–110
Yamaguchi M, Ozaki K (1990) Effect of the new zinc compound beta-alanyl-L-histidinato zinc on bone metabolism in elderly rats. Pharmacology 41:345–349
Yamaguchi M, Miwa H (1991) Stimulatory effect of beta-alanyl-L-histidinato zinc on bone formation in tissue culture. Pharmacology 42:230–240
Yamaguchi M, Ohtaki J (1991) Effect of beta-alanyl-L-histidinato zinc on osteoblastic MC3T3-E1 cells: increases in alkaline phosphatase and proliferation. Pharmacology 43: 225–232
Yamaguchi M, Oishi H, Suketa Y (1987) Stimulatory effect of zinc or bore formation in tissue culture. Biochem Pharmacol 36:4001–4012
Yamaguchi M, Segawa Y, Shimokawa N, Tsuzuike N, Tagashira E (1992) Inhibitory effect of beta-alanyl-L-histidinato zinc on bone resorption in tissue culture. Pharmacology (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Segawa, Y., Tsuzuike, N., Itokazu, Y. et al. β-Alanyl-L-histidinato zinc prevents hydrocortisone-induced disorder of bone metabolism in rats. Res. Exp. Med. 192, 317–322 (1992). https://doi.org/10.1007/BF02576288
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02576288