Abstract
We studied the cardiac uptake of 2-14C-D-deoxy-glucose (14C-DG) in 6 dogs subjected to a 15 min left anterior descending coronary artery (LAD) occlusion followed by 30 min reflow. Coronary arterial flows during occlusion were determined with46Sc microspheres. After 30 min reflow, 50 μCi14C-DG were given intravenously to determine cardiac uptake. The animal was killed 45 min later and46Sc counts/min were determined in gamma and beta counters. Quenching was determined with non radioactive blood for both isotopes in the beta counter. Contribution of46Sc counts/min to the14C channel was subtracted.14C uptake was in DPM/g and was higher in the cardiac ischemic regions of four of the six dogs. In three of the six animals there was an inverse curvilinear relation between14C-DG cardiac uptake and occlusion flow. This observation was further confirmed by high resolution autoradiography, indicating that enhanced cardiac uptake of radiolabeled DG may be a useful positron emission tomographic marker for reversible myocardial ischemia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bateman TM, Czer LSC, Gray RJ (1983) Transient pathologic Q waves during acute ischemic events: An electrocardiographic correlate of stunned but viable myocardium. Am Heart J 106:1421–1426
Bianco JA, Pape LA, Alpert JS, Zheng M, Hnatowich D, Goodman MM, Knapp FF (1984) Accumulation of radioiodinated 15-(p-iodophenyl)-6-tellurapentodecanoic acid in ischemic myocardium during acute coronary occlusion and reperfusion. J Am Coll Cardiol 4:80–87
Braunwald E, Kloner RA (1982) The stunned myocardium: Prolonged, postischemic ventricular dysfunction. Circulation 66:1146–1149
Brunken R, Tillisch J, Schwaiger M, Child JS, Marshall R, Mandelkern M, Phelps ME, Schelbert HR (1986) Regional perfusion, glucose metabolism, and wall motion in patients with chronic electrocardiographic Q wave infarctions: Evidence for persistence of viable tissue in some infarct regions by positron emission tomography. Circulation 73:951–963
Camici P, Araujo LI, Spinks T, Lammertsma AA, Kaski JC, Shea MJ, Selwyn AP, Jones T, Maseri A (1986) Increased uptake of F-18-fluorodeoxyglucose in postischemic myocardium of patients with exercise-induced ischemia. Circulation 74:81–88
Challis RAJ, Hayes DJ, Radda GK (1986) An investigation of arterial insufficiency in the rat hindlimb. Correlation of skeletal muscle blood flow and glucose utilization in vivo. Biochem J 240:395–401
DeBoer LWV, Ingwall JS, Kloner RA, Braunwald E (1980) Prolonged derangements in canine myocardial purine metabolism after a brief coronary artery occlusion not associated with anatomic evidence of necrosis. Proc Natl Acad Sci USA 77:5471–5475
Ganz W, Geft I, Maddahi J, Berman D, Charuzi Y, Shah PK, Swan HJC (1983) Nonsurgical reperfusion in evolving myocardial infarction. J Am Coll Cardiol 1:1247–1253
Heyman MA, Payne BD, Hoffman JE, Rudolph AM (1977) Blood flow measurements with radionuclide-labeled particles. Prog Cardiovasc Dis 20:55–79
Ishida H, Sheck R, Bridge JHB, Barry WH (1986) Changes in K uptake, Na and K contents, and (ATP) after recovery from metabolic inhibition in cultured heart cells. Circulation [Suppl II] 74:434 (abstr)
Kobayashi Y, Maudsley DV (1974) Biological applications of liquid scintillation counting. Academic Press, New York
Krivokapich J, Huang SC, Phelps ME, Barrio JR, Watanabe CR, Selin CE, Shine KI (1982) Estimation of rabbit myocardial metabolic rate for glucose using fluorodeoxyglucose. Am J Physiol 243:H884-H895
Liedtke AJ (1981) Alterations of carbohydrate and lipid metabolism in the acutely ischemic heart. Prog Cardiovasc Dis 23:321–336
Marshall RC, Tillisch JH, Phelps ME, Huang SC, Carson R, Henze E, Schelbert HR (1983) Identification and differentiation of resting myocardial ischemia and infarction in man with positron computed tomography, F-18-labeled fluorodeoxyglucose and N-13 ammonia. Circulation 67:766–778
Mochizuki S, Neely JR (1980) Energy metabolism during reperfusin following ischemia. J Physiol (Paris) 76:805
Myears DW, Sobel BE, Bergmann SR (1987) Substrate use in ischemic and reperfused canine myocardium: Quantitative considerations. Am J Physiol 253: H107-H114
Neurohr KJ, Gollin G, Neurohr JM, Rothman DL, Shulman RG (1984) Carbon-13 nuclear magnetic resonance studies of myocardial glycogen metabolism in live guinea pigs. Biochemistry 23:5029–5035
Oppie LH (1976) Effects of regional ischemia on metabolism of glucose and fatty acids: Relative rates of aerobic and anaerobic energy production during myocardial infarction and comparison with effects of anoxia. Circ Res [Suppl 1] 38:152–168
Paulin S (1986) Angiographic and radiologic comments on thrombolytic therapy in acute myocardial infarction. Cardiovasc Intervent Radiol 9:258–260
Phelps ME, Hoffman HJ, Selin CE, Huang SC, Robinson G, MacDonald N, Schelbert HR, Kuhl DE (1978) Investigation of F-18-fluorodeoxyglucose for the measure of myocardial glucose metabolism. J Nucl Med 19:1311–1319
Ratib O, Phelps ME, Huang SC, Henze E, Selin CE, Schelbert HR (1982) Positron tomography with deoxyglucose for estimating local myocardial glucose metabolism. J Nucl Med 23:577–586
Reimer KA, Hill ML, Jennings RB (1981) Prolonged depletion of ATP and of adenine nucleotide pool due to delayed resynthesis of adenine nucleotides following reversible myocardial ischemic injury in dogs. J Mol Cell Cardiol 13:229–239
Reimer KA, Jennings RB (1979) The “wavefront phenomenon” of myocardial ischemic cell death. II. Transmural progression of necrosis within the framework of ischemic bed size (myocardium at risk) and collateral flow. Lab Invest 40:633–642
Rippe JM, Pape LA, Alpert JS, Ockene IS, Paraskos JA, Kotilainen P, Anas J, Webster W (1982) Studies of systolic mechanics and diastolic behavior of the left ventricle in the trained racing greyhound. Basic Res Cardiol 77:619–644
Schwaiger M, Hansen HW, Vinten-Johansen J, Ellison DJ, Yeatman LA, Schelbert HR (1983) Regional myocardial function and metabolism after reperfusion in chronic dog experiments. J Am Coll Cardiol 1:688
Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakaruda O, Shinohara M (1977) The C-14 deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28:897–916
Tillisch J, Brunken R, Marshall R, Schwaiger M, Mandelkern M, Phelps M, Schelbert H (1986) Reversibility of cardiac wall motion abnormalities predicted by positron tomography. New Engl J Med 314:884–888
Wilson RA, Shea M, DeLandsheere C, Deanfeld J, Lammertsma AA, Jones T, Selwyn AP (1987) Rubidium-82 myocardial uptake and extraction after transient ischemia: PET characteristics. J Comput Assist Tomogr 11:60–66
Yonekura Y, Brill AB, Som P, Yamamoto K, Srivastava SC, Iwai J, Elmaleh DR, Livni E, Strauss HW, Goodman MM, Knapp FF Jr (1985) Regional myocardial substrate uptake in hypertensive rats: A quantitative autoradiographic measurement. Science 277:1494–1496
Author information
Authors and Affiliations
Additional information
This work was presented in part at the 36th Annual Scientific Sessions, American College of Cardiology, March 8–12, 1987, New Orleans, Louisiana. Supported by NIH ROI Grant No. HL33514-01A1
Rights and permissions
About this article
Cite this article
Bianco, J.A., Bakanauskas, J., Carlson, M. et al. Augmented uptake of 2-C-14-v-deoxyglucose in reversibly-injured myocardium. Eur J Nucl Med 13, 557–562 (1988). https://doi.org/10.1007/BF02574767
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02574767