Summary
To take advantage of both differential digestion by hypochlorite and solvent extraction, we used dispersions of sodium hypochlorite solution and chloroform in the recovery of microbial PHB. The treatment with hypochlorite alone caused such severe degradation and the molecular weight decreased drastically with increasing hypochlorite concentration. However, using the dispersion, the degradation of PHB was markedly diminished owing to theshielding effect of chloroform. In this case, we could obtain PHB of above 97% purity with a number average molecular weight of 1,000,000 comparable to the original molecular weight of 1,200,000.
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Hahn, S.K., Chang, Y.K., Kim, B.S. et al. The recovery of poly(3-hydroxybutyrate) by using dispersions of sodium hypochlorite solution and chloroform. Biotechnol Tech 7, 209–212 (1993). https://doi.org/10.1007/BF02566149
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DOI: https://doi.org/10.1007/BF02566149