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Development of an apparatus for cultivation of anaerobic microorganisms

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Biotechnology Techniques

Summary

A novel apparatus with an L-shaped test tube was developed for anaerobic cell cultivation. Anaerobic condition was achieved without the rigorous gassing with CO2 during the various stages of medium preparation. dispensation and cell inoculation. The growth of both moderate and strict anaerobes in this apparatus were similar to those obtained with the glove box method.

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Authors and Affiliations

  1. Institute of Applied Biochemistry, University of Tsukuba, 305, Tsukuba, Ibaraki, Japan

    H. Tanaka, M. Nakanishi, J. C. Ogbonna & Y. Ashihara

Authors
  1. H. Tanaka
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  2. M. Nakanishi
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  3. J. C. Ogbonna
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  4. Y. Ashihara
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Tanaka, H., Nakanishi, M., Ogbonna, J.C. et al. Development of an apparatus for cultivation of anaerobic microorganisms. Biotechnol Tech 7, 189–192 (1993). https://doi.org/10.1007/BF02566145

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  • DOI: https://doi.org/10.1007/BF02566145

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