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Direct somatic embryogenesis in leaves ofCichorium

A histological and SEM study of early stages

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Summary

Leaves from 2-month-old in vitro grown plantlets of a clone ofCichorium placed in agitated liquid induction medium at 35°C in the dark produce embryoids after 5 days of culture, without synchronization. Vascular sheath parenchyma cells react first, but every mesophyll cell is potentially embryogenic. Single cells show an early patchy callosic wall and undergo dedifferentiation. With SEM the cells of those proembryoids just emerging through the epidermis are seen to be linked by a fibrillar network, the nature of which is discussed. Four FITC-labelled lectins were tested; only DBA shows embryogenic specificity.

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Abbreviations

SEM:

scanning electron microscopy

PBS:

phosphate buffer saline

PAS:

periodic acid-Schiff reaction

FITC:

fluorescein isothiocyanate

Con A:

concanavallin agglutinin fromCanavallia ensiformis

DBA:

Dolichos biflorus agglutinin

UEA:

Ulex europaeus agglutinin

WGA:

wheat germ agglutinin

NAA:

1-naphtaleneacetic acid

2iP:

6-dimethylallylamino-purine

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Authors and Affiliations

  1. Laboratoire de Physiologie de la Différenciation et Biotechnologies Végétales, Université des Sciences et Techniques de Lille Flandres-Artois, SN2, F-59655, Villeneuve d'Ascq Cedex, France

    T. Dubois, M. Guedira, J. Dubois & J. Vasseur

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  1. T. Dubois
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  2. M. Guedira
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  3. J. Dubois
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  4. J. Vasseur
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Dubois, T., Guedira, M., Dubois, J. et al. Direct somatic embryogenesis in leaves ofCichorium . Protoplasma 162, 120–127 (1991). https://doi.org/10.1007/BF02562555

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  • DOI: https://doi.org/10.1007/BF02562555

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