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Prostaglandin-Induced changes in calcium uptake and cAMP production in osteoblast-like cells: Role of protein kinase C

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Summary

Phorbol esters were used to evaluate the putative effect of protein kinase C (PKC) activation on prostaglandin E2 (PGE2)-induced increases in calcium uptake and cAMP production in the human osteoblastic osteosarcoma cell line, Saos-2. The cells were pretreated for 15 min with phorbol myristate acetate (PMA) followed by a 5 min incubation with PGE2. Calcium uptake was measured with45Ca and cAMP by radioimmunoassay. A significant increase in calcium uptake was noted in the PGE-treated cells compared with controls and preincubation with the PMA caused a significant decrease in this response. Preincubation with PMA also inhibited the PGE2-induced increase in cAMP under identical conditions. The effect of PMA on the cAMP response was not influenced by the addition of a phosphodiesterase inhibitor. PMA had no effect on the basal levels of either calcium uptake or cAMP production. Likewise, the inactive phorbol esters, phorbol 12,13-didecanoate (PDD) and 4 α-phorbol 12-myristate, 13-acetate (4alpha), had no effect on either basal levels of these parameters or on the PGE2-induced increases. These results suggest that PKC is involved in the down-regulation of PGE2-induced increases in calcium uptake and cAMP production in the Saos-2 osteoblastic cell line.

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Authors and Affiliations

  1. Department of Endodontics, School of Dental Medicine, State University of New York at Buffalo, Foster Hall, 14214, Buffalo, New York, USA

    S. Hagel-Bradway

  2. Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Foster Hall, 14214, Buffalo, New York, USA

    S. Hagel-Bradway, D. N. Tatakis & R. Dziak

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  1. S. Hagel-Bradway
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  2. D. N. Tatakis
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  3. R. Dziak
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Hagel-Bradway, S., Tatakis, D.N. & Dziak, R. Prostaglandin-Induced changes in calcium uptake and cAMP production in osteoblast-like cells: Role of protein kinase C. Calcif Tissue Int 48, 272–277 (1991). https://doi.org/10.1007/BF02556379

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  • DOI: https://doi.org/10.1007/BF02556379

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