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Proteins of the mineral compartment of bovine fetal enamel share common antigenic determinants with serum proteins

  • Molecular and Cellular Biology
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Summary

Two fractions were separated from the proteins of the mineral compartment of bovine developing enamel on the basis of their affinity for the lectin concanavalin-A. A monoclonal antibody was prepared by the hybridoma technique agains the Con-A-binding fraction. This antibody and a commercial polyclonal antibody against bovine serum albumin were used to examine the relationship between those proteins, serum albumin and alpha-2HS glycoprotein, two proteins concentrated within dentin and bone matrices. The Con-A-unbound fraction reacted with the anti-albumin antibody and the antibody against the Con-A-binding fraction recognized the alpha-2HS glycoprotein. These data fully support the presence of significant levels of proteins related to serum components in the mineral compartment of developing enamel matrix.

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Authors and Affiliations

  1. U. 225 INSERM, UFR d'Odontologie, Université de Nantes, Place Alexis Ricordeau, 44042, Nantes, France

    J. Menanteau, S. Dajean & O. Laboux

  2. U.211 INSERM, UFR de Médecine-Pharmacie, Université de Nantes, Rue Gaston Veil, 44035, Nantes, France

    J. Aubry

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  1. J. Menanteau
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  2. S. Dajean
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  3. O. Laboux
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  4. J. Aubry
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Menanteau, J., Dajean, S., Laboux, O. et al. Proteins of the mineral compartment of bovine fetal enamel share common antigenic determinants with serum proteins. Calcif Tissue Int 47, 251–255 (1990). https://doi.org/10.1007/BF02555927

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  • DOI: https://doi.org/10.1007/BF02555927

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