Abstract
The spectrum of plasma proteins present in human cortical bone and permanent dentine has been determined. One plasma glycoprotein, theαHS-glycoprotein, was found to be present at a high concentration in both bone and dentine and was shown to be concentrated in the mineralized tissues with respect to the other plasma proteins by factors of between 30 and 100. In this respect theαHS-glycoprotein is analogous to the G2B-glycoprotein and α-glycoprotein of bovine and rabbit b one, respectively. The binding ofαHS-glycoprotein and albumin to calcium phosphates generated within serum samples has been studied at different serum: precipitate ratios. In each case all theαHS-glycoprotein was removed from the samples and theαHS-glycoprotein was concentrated with respect to albumin by factors ranging from 370 at the highest serum: precipitate ratio to 25 at the lowest ratio. The plasmaαHS-glycoprotein concentrations of patients with Paget's disease of bone were shown to be substantially lower than the normal range, with significant negative correlation between theαHS-glycoprotein concentration and the plasma alkaline phosphatase activity.
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Ashton, B. A., Triffitt, J. T., Herring, G. M.: Isolation and partial characterization of a glycoprotein from bovine cortical bone. Europ. J. Biochem.45, 525–533 (1974).
Blumenthal, N. C., Betts, F., Posner, A. S.: Effect of carbonate and biological macromolecules on formation and properties of hydroxyapatite. Calcif. Tiss. Res.18, 81–90 (1975).
Dickson, I. R., Poole, A. R., Veis, A.: Localization of plasmaαHS-glycoprotein in mineralizing human bone. Nature (Lond.)256, 430–432 (1975)
Fiske, C. H., Subba Row, Y.: The colorimetric determination of phosphorus. J. Biol. Chem.66, 375–79 (1925)
Franck, W. A., Bress, N. M., Singer, F. R., Krane, S. M.: Rheumatic manifestations of Paget's disease of bone. Amer. J. Med.56, 592–597 (1974)
Herring, G. M., Ashton, B. A., Chipperfield, A. R.: The isolation of soluble proteins, glycoproteins, and proteoglycans from bone. Prep. Biochem.4, 179–190 (1974).
Jones, I. L., Leaver, A. G.: Studies on the minor components of the organic matrix of human dentine. Archs. oral Biol.19, 371–380 (1974).
Kind, P. R. N., King, E. J.: Estimation of plasma phosphatase by the determination of hydrolysed phenol with amino-antipyrine. J. clin. Path.7, 322–326 (1954).
Lipp, W.: Blood serum protein and the mineralization of bone ground substance. Histochemie9, 339–353 (1967).
Mancini, G., Carbonara, A. O., Heremans, J. F.: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry2, 235–254 (1965)
Neuman, W. F.: The presence of a soluble “regulator” phase (secondary calcium phosphate) in bone. In: Calcium-regulating hormones (Talmage, R. V., Owen, M., Parsons, J. A., eds.), pp. 297–302. Amsterdam: Excerpta Medica, 1975
Oss, C. J., van, Gillman, C. F., Bronson, P. M., Border, J. R.: Opsonic properties of human serum alpha-2 HS-glycoprotein. Immun. Commun.3, 329–335 (1974)
Ouchterlony, O.: In vitro method for testing the toxin-producing capacity of diphtheria bacteria. 8th Scandinavian Pathological Congress 1947, Acta path. microbiol. scand.25, 186–195 (1948)
Owen, M., Triffitt, J. T.: Plasma glycoproteins and bone. In: Calcium, parathyroid hormone and the calcitonins (Talmage, R. V., Munson, P. L., eds.), pp. 316–326. Amsterdam: Excerpta Medica 1972
Owen, M., Triffitt, J. T.: Extravascular albumin in bone tissue. J. Physiol. (Lond.)257, 293–307 (1976)
Owen, M., Triffitt, J. T. Melick, R. A.: Albumin in bone. In: Hard tissue, growth repair and mineralization, Ciba Foundn. Symp. 11, pp. 263–285 (1973)
Schmid, K., Bürgi, W.: Preparation and properties of the human plasmaα. Biochem. biophys. Acta (Amst.)47, 440–453 (1961)
Termine, J. D.: Mineral chemistry and skeletal biology. Clin. Orthop.85, 207–241 (1972)
Thomas, M., Leaver, A. G.: Identification and estimation of plasma proteins in human dentine. Arch. oral. Biol.20, 217–218 (1975)
Triffitt, J. T., Owen, M.: Studies on bone matrix glycoproteins. Incorporation of (1-14C) glucosamine and plasma (14C) glycoprotein into rabbit cortical bone. Biochem. J.136, 125–134 (1973)
Triffitt, T. T., Gebauer, U., Ashton, B. A., Owen, M. E., Reynolds, T. T.: Origin of plasmaαHS-glycoprotein and its accumulation in bone. Nature (Lond.)262, 226–227 (1976)
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Ashton, B.A., Höhling, H.J. & Triffitt, J.T. Plasma proteins present in human cortical bone: Enrichment of theαHS-glycoprotein. Calc. Tis Res. 22, 27–33 (1977). https://doi.org/10.1007/BF02010343
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DOI: https://doi.org/10.1007/BF02010343