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Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma

  • Original Contributions
  • Published:
Diseases of the Colon & Rectum

Abstract

To gain a better understanding of the biologic development of rectal adenicarcinomas, the authors evaluated the level ofras gene protein product (p21) in the available material of 74 Dukes'B adenocarcinomas, 64 Dukes' C adenocarcinomas, and 60 lymph-node metastases resected at the University of Chicago Medical Center between 1965 and 1981. Pathologic slides and archival paraffin blocks were retrieved for confirmation of the original diagnosis and measurement of p21 content. P21 titers were obtained using the RAP-5 monoclonal antibody in a semiquantitative immunohistochemical assay. Titer was expressed as the highest dilution giving definitive staining using the avidinbiotin peroxidase method. The analysis indicated that a higher percentage of Dukes' stage C rectal adenocarcinomas had high (≥1∶40,000) p21 titers than Dukes' B adenocarcinomas (68.8vs. 51.4 percent, respectively,P<0.05). In view of recent data suggesting thatras oncogene expression confers invasive and metastatic capabilities to NIH 3T3 cells, the authors believe this study offers evidence that overexpression ofras oncogene with overproduction of p21 protein product may be an important prerequisite for the acquisition of metastatic capabilities in the early stages of colon cancer.

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Authors and Affiliations

  1. Department of Surgery, University of Chicago, 5841 South Maryland Avenue, 60637, Chicago, Illinois

    Fabrizio Michelassi M.D., Francesca Erroi M.D., Manuela Roncella M.D. & George E. Block M.D.

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  1. Fabrizio Michelassi M.D.
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  2. Francesca Erroi M.D.
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  3. Manuela Roncella M.D.
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  4. George E. Block M.D.
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Supported in part by Award#87-100 of the American Cancer Society.

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Michelassi, F., Erroi, F., Roncella, M. et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum 32, 665–668 (1989). https://doi.org/10.1007/BF02555770

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  • DOI: https://doi.org/10.1007/BF02555770

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