Summary
In this investigation we examined the chemotaxis of muscle-derived mesenchymal cells from neonatal rats to partially purified extracts of demineralized bone matrix with osteoinductive properties. Using the modified Boyden chamber assay and muscle-derived mesenchymal cells obtained from neonatal Long-Evans rats, we tested the chemotactic properties of the 4 M guanidine-HCl extract from demineralized rat bone matrix and fractions thereof that were derived from sequential Sepharose CL-6B, TSK-3000 and HPLC-C18 chromatography. We have identified that those fractions exhibiting chemotactic properties were also osteoinductive. Therefore, demineralized bone matrix serves as its own soluble signal and insoluble substratum in the inductive process leading to endochondral bone formationin vivo.
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References
Urist MR (1965) Bone formation by autoinduction. Science 150:893–899
Reddi AH, Huggins CB (1972) Biochemical sequences in the transformation of normal fibroblasts in adolescent rats. Proc Natl Acad Sci USA 69:1601–1605
Reddi AH (1985) Regulation of bone differentiation by local and systemic factors. In: Peck WA (ed) Bone and Mineral Research 3:27–47, Elsevier
Somerman M, Hewitt AT, Varner HH, Schiffmann E, Termine J, Reddi AH (1983) Identification of a bone matrix-derived chemotactic factor. Calcif Tissue Int 35:481–485
Sampath TK, Reddi AH (1981) Dissociative extraction and reconstitution of extracellular matrix components involved in local bone differentiation. Proc. Nat Acad Sci USA 78:7599–7603
Postlethwaite AE, Synderman R, Kang AH (1976) Chemotactic attraction of human fibroblasts to a lymphocyte-derived factor. J Exp Med 144:1188–1203
Zigmond SH, Hirsch JG (1973) Leukocyte locomotion and chemotaxis: new methods for evaluation and demonstration of a cell-derived chemotactic factor. J Exp Med 137:387–410
Sampath TK, Reddi AH (1983) Homology of bone-inductive proteins from human, monkey, bovine and rat extracellular matrix. Proc Natl Acad Sci USA 80:6591–6595
Grotendorst GR, Seppa HEJ, Kleinman HK, Martin GR (1981) Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci USA 6:3669–3672
Grotendorst GR (1984) Alteration of the chemotactic response of NIH/3T3 cells to PDGF by growth factors, transformation, and tumor promotors. Cell 36:279–285
Seppa H, Grotendorst G, Seppa S, Schiffmann E, Martin GR (1982) Platelet-derived growth factor is chemotactic for fibroblasts.
Venkatasubramanian K, Solursh M (1984) Chemotactic behavior of myoblasts. Devel Biol 104:428–433
Sampath TK, Reddi AH (1984) Importance of geometry of the extracellular matrix in endochondral bone differentiation. J Cell Biol 98:2192–2197
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Landesman, R., Reddi, A.H. Chemotaxis of muscle-derived mesenchymal cells to bone-inductive proteins of rat. Calcif Tissue Int 39, 259–262 (1986). https://doi.org/10.1007/BF02555215
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DOI: https://doi.org/10.1007/BF02555215