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Assay of bone resorption in vivo with3H-tetracycline

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Abstract

3H-Tetracycline (3H-TC) was used to quantify resorption in whole bones of growing rats and dogs. After repeated isotopic labeling of actively growing embryos or neonates,3H-TC was observed to be distributed homogeneously and in equilibrium with45Ca. A rapid and large loss of3H-TC and a small loss of45Ca occurred during the early weeks of rapid bone growth, suggesting thatabsolute amounts of45Ca resorbed from bone, as reflected by losses of3H-TC, are five to ten times greater than thenet amounts of45Ca lost from bone. Minimal loss of3H-TC occurred due to nonspecific physicochemical exchange in vivo or in vitro (5%) except with nonradioactive tetracycline, and3H-TC was not greatly exchanged or reused (10%) in vivo. The data are considered in terms of local and systemic conservation of calcium.

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  1. Division of Orthopedic Surgery, Department of Surgery, Case Western Reserve University School of Medicine, Cleveland, Ohio

    LeRoy Klein & Kenneth Van Jackman

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  1. LeRoy Klein
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Klein, L., Van Jackman, K. Assay of bone resorption in vivo with3H-tetracycline. Calc. Tis Res. 20, 275–290 (1976). https://doi.org/10.1007/BF02546415

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