Abstract
The recessive genesmi andgl in the homozygous state determine, among other phenotypic effects, osteopetrosis in the house mouse. From a stock carryingmi derived from Grüneberg (1963) themi gene was bred into the standard CBA/H inbred strain. Microphthalmic mice of these two stocks and their hybrids were treated as newborn by intraperitoneal injection and at weaning or maturity by intravenous injection of cell suspensions containing hematopoietic stem cells from phenotypically normal mice. Resolution of much of the osteopetrosis but not the other phenotypic effects occurred within a few months in the majority of cases, provided syngeneic or H-2 compatible allogeneic cells were given: it did not occur spontaneously or on giving H-2 incompatible cells or on giving compatible material by an inappropriate route. The results accord with hypotheses that (1) osteoclasis of scaffold-type woven bone is impaired inmi mi, (2) that osteoclastic cells are derived through circulating monocytes from hematopoietic stem cells, and (3) inmi mi this defect can be overcome by a transplant of normal hemopoietic stem cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnes, D. W. H., Loutit, J. F., Sansom, J. M.: Histocompatible cells for the resolution of osteopetrosis in microphthalmic mice. Proc. roy. Soc. B188, 501–505 (1975)
Barnicot, N. A.: Studies on the factors involved in bone absorption. The effects of subcutaneous transplantation of bones of the grey lethal house mouse with normal hosts and of normal bones with grey lethal hosts. Amer. J. Anat.68, 497–531 (1941)
Friedenstein, A. J.: Determined and inducible osteogenic precursor cells. In: Hard Tissue Growth Repair and Remineralization. Ciba Foundation Symposium 11 (new Series), pp. 169–185. Amsterdam: Elsevier 1973
Göthlin, G., Ericsson, J. L. E.: On the histogenesis of the cells in fracture callus. Electron microscopic autoradiographic observations in parabiotic rats and studies on labelled monocytes. Virchows Arch. Abt. B12, 318 (1973)
Grüneberg, H.: The pathology of development, Chap. 4. Oxford: Blackwell’s Scientific Publication 1963
Hancox, N. M.: The biology of bone, Chap. 12. Cambridge: University Press 1972
Jee, W. S. S., Nolan, P. D.: Origin of osteoclasts from fusion of phagocytes. Nature (Lond.)200, 225–226 (1963)
Lendrum, A. C., Slidders, W., Fraser, D. S.: A study of amyloidosis and diabetic fibrous vasculosis with new staining methods. J. clin. Path.25, 373–396 (1972)
Little, Kitty: Bone behaviour, p. 166. London: Academic Press 1973
Micklem, H. S., Loutit, J. F.: Tissue grafting and radiation, p. 82. New York: Academic Press 1966
Murphy, Helen, M.: A review of inherited osteopetrosis in the mouse. Clin. Orthop.65, 97–109 (1969)
Murphy, Helen, M.: The osteopetrotic syndrome in the microphthalmic mouse. Calcif. Tiss. Res.13, 19–26 (1973)
Osipov, V. V., Konyukhov, B. K.: Analysis of the development of osteopetrosis in mice of the mutant strain microphthalmia. Soviet Genetics Genetika3, No. 9, 126–135 (1967)
Owen, M.: The origin of bone cells. Int. Rev. Cytol.28, 213–238 (1970)
Walker, D. G.: Osteopetrosis in mice cured by temporary parabiosis. Science180, 975 (1973)
Note added in proof
Walker, D. G.: Control of bone resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through use of bone marrow and splenic transplants. J. exp. Med.142, 651–663 (1975)
Walker, D. G.: Bone resorption restored in osteopetrotic mice by transplants of normal bone marrow and spleen cells. Science190, 784–785 (1975)
Walker, D. G.: Spleen cells transmit osteopetrosis in mice. Science190, 785–787 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Loutit, J.F., Sansom, J.M. Osteopetrosis of microphthalmic mice—A defect of the hematopoietic stem cell?. Calc. Tis Res. 20, 251–259 (1976). https://doi.org/10.1007/BF02546413
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02546413