Abstract
Bone from rats sacrified at 2, 5, and 95 weeks of age following45Ca and3H-tetracycline labelingin utero or postnatally was placed in solutions of physiological saline, nonradioactive calcium, or tetracycline. This procedure was designed to determine the availability for exchange of45Ca and3H-tetracycline from bone mineral in vitro. With time after labeling a gradual decrease occurred in both the nonspecific loss and specific exchange of45Ca and3H-tetracycline.45Ca and3H-tetracycline in bone could be partially exchanged in vitro with their respective nonradioactive counterparts. Each isotope could be partially exchanged, independently of the other isotope. Nonradioactive calcium increased the exchange of45Ca while suppressing the release of3H-tetracycline. Molecular3H-tetracycline exchange occurred to a greater degree than ionic45Ca exchange and was associated with a small loss of45Ca. The molecular exchange of3H-tetracycline with bulk tetracycline did not induce any significant loss of tritium from3H-tetracycline.
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Klein, L., Reilly, D.T. Concurrent exchange of45Ca and3H-tetracycline from rat bone in vitro. Calc. Tis Res. 20, 229–234 (1976). https://doi.org/10.1007/BF02546411
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DOI: https://doi.org/10.1007/BF02546411