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Total and peroxisomal oxidation of various saturated and unsaturated fatty acids in rat liver, heart and m. quadriceps

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Lipids

Abstract

Rates of total and peroxisomal fatty acid oxidation were estimated from the production of14C-labeled CO2 and acid-soluble products from differently labeled [14C]fatty acids, in the absence and presence of antimycinrotenone, in homogenates of liver, heart and m. quadriceps. Total and peroxisomal oxidation rates of palmitic, oleic and linoleic acid were 3–4 times higher than those of arachidonic and adrenic acid which had higher oxidation rates than those of lignoceric and erucic acid. The peroxisomal contribution to the oxidation of the last fatty acids was similar to or higher than that of palmitic acid. For all fatty acids tested in these tissues, the mitochondrial contribution to β-oxidation was higher than the peroxisomal contribution.

Production of14CO2 and14C-labeled, acid-soluble metabolites from [13-14]arachidonic acid indicated that polyunsaturated fatty acids can be chain-shortened beyond their double bonds in m. quadriceps and heart as well as in liver. Although 2,4-dienoyl-CoA reductase requires NADPH, addition of this coenzyme did not influence arachidonic acid oxidation. Arachidonic acid oxidation was inhibited by palmitic acid in mitochondria and peroxisomes, but arachidonic acid had only a slight effect on palmitic acid oxidation.

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Abbreviations

CoA:

coenzyme A

EDTA:

ethylenediamine tetraacetic acid

HCl:

hydrochloric acid

NAD:

nicotinamide adenine dinucleotide

NADPH:

reduced nicotinamide adenine dinucleotide phosphate

References

  1. Dommes, P., Dommes, V., and Kunau, W-H. (1983)J. Biol. Chem. 258, 10846–10852.

    PubMed  CAS  Google Scholar 

  2. Dommes, V., Baumgart, C., and Kunau, W-H. (1981)J. Biol. Chem. 256, 8259–8262.

    PubMed  CAS  Google Scholar 

  3. Chu, C., Kushner, L., Cuebas, D., and Schulz, H. (1984)Biochem. Biophys. Res. Commun. 118, 162–167.

    Article  PubMed  CAS  Google Scholar 

  4. Clejan, S., and Schulz, H. (1986)Arch. Biochem. Biophys. 246, 820–828.

    Article  PubMed  CAS  Google Scholar 

  5. Cuebas, D., and Schulz, H. (1982)J. Biol. Chem. 257, 14140–14144.

    PubMed  CAS  Google Scholar 

  6. Kärki, T., Hakkola, E., Hassinen, I.E., and Hiltunen, J.K. (1987)FEBS Lett. 215, 228–232.

    Article  PubMed  Google Scholar 

  7. Kimura, C., Kondo, A., Koeda, N., Yamanaka, H., and Mizugaki, M. (1984)J. Biochem. 96, 1463–1469.

    PubMed  CAS  Google Scholar 

  8. Stoffel, W., Ditzer, R., and Caesar, H. (1964)Hoppe Seyler's Z. Physiol. Chem. 339, 167–181.

    PubMed  CAS  Google Scholar 

  9. Yang, S-Y., Cuebas, D., and Schulz, H. (1986)J. Biol. Chem. 261, 12238–12243.

    PubMed  CAS  Google Scholar 

  10. Smeland, T.E., Li, J., Chu, C., Cuebas, D., and Schulz, H. (1989)Biochem. Biophys. Res. Commun. 160, 988–992.

    Article  PubMed  CAS  Google Scholar 

  11. Hiltunen, J.K., Osmundsen, H., and Bremer, J. (1983)Biochim. Biophys. Acta 752, 223–232.

    PubMed  CAS  Google Scholar 

  12. Osmundsen, H., Cervenka, J., and Bremer, J. (1982)Biochem. J. 208, 749–757.

    PubMed  CAS  Google Scholar 

  13. Alexson, S.E.H., and Cannon, B. (1984)Biochim. Biophys. Acta 796, 1–10.

    PubMed  CAS  Google Scholar 

  14. Bremer, J., and Norum, K.R. (1982)J. Lipid Res. 23, 243–256.

    PubMed  CAS  Google Scholar 

  15. Hiltunen, J.K., Kärki, T., Hassinen, I.E., and Osmundsen, H. (1986)J. Biol. Chem. 261, 16484–16493.

    PubMed  CAS  Google Scholar 

  16. Hovik, R., and Osmundsen, H. (1987)Biochem. J. 247, 531–535.

    PubMed  CAS  Google Scholar 

  17. Neat, C.E., Thomassen, M.S., and Osmundsen, H. (1981)Biochem. J. 196, 149–159.

    PubMed  CAS  Google Scholar 

  18. Osmundsen, H., Neat, C.E., and Borrebaek, B. (1980)Int. J. Biochem. 12, 625–630.

    Article  PubMed  CAS  Google Scholar 

  19. Osmundsen, H. (1982)Annals N.Y. Acad. Sci. 386, 13–29.

    Article  CAS  Google Scholar 

  20. Osmundsen, H. (1982)Int. J. Biochem. 14, 905–914.

    Article  PubMed  CAS  Google Scholar 

  21. Christensen, E., Hagve, T-A., and Christophersen, B.O. (1986)Biochim. Biophys. Acta 879, 313–321.

    PubMed  CAS  Google Scholar 

  22. Hagve, T-A., and Christophersen, B.O. (1986)Biochim. Biophys. Acta 875, 165–173.

    PubMed  CAS  Google Scholar 

  23. Reubsaet, F.A.G., Veerkamp, J.H., Bukkens, S.G.F., Trijbels, J.M.F., and Monnens, L.A.H. (1988)Biochim. Biophys. Acta 958, 434–442.

    PubMed  CAS  Google Scholar 

  24. Veerkamp, J.H., and van Moerkerk, H.T.B. (1986)Biochim. Biophys. Acta 875, 301–310.

    PubMed  CAS  Google Scholar 

  25. Veerkamp, J.H., van Moerkerk, H.T.B., Glatz, J.F.C., Zuurveld, J.G.E.M., Jacobs, A.E.M., and Wagemakers, A.J.M. (1986)Biochem. Med. Metab. Biol. 35, 248–259.

    Article  PubMed  CAS  Google Scholar 

  26. Kondrup, J., and Lazarow, P.B. (1985)Biochim. Biophys. Acta 835, 147–153.

    PubMed  CAS  Google Scholar 

  27. Handler, J.A., and Thurman, R.G. (1988)Eur. J. Biochem. 176, 477–484.

    Article  PubMed  CAS  Google Scholar 

  28. Vamecq, J., Schepers, L., Parmentier, G., and Mannaerts, G.P. (1987)Biochem. J. 248, 603–607.

    PubMed  CAS  Google Scholar 

  29. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  30. Gregory, R.B., and Berry, M.N. (1986)Eur. J. Biochem. 160, 645–649.

    Article  PubMed  CAS  Google Scholar 

  31. MacDonald, R.S., and Swan, P.B. (1986)Biochim. Biophys. Acta 880, 153–160.

    PubMed  CAS  Google Scholar 

  32. Nemali, M.R., Usuda, N., Reddy, M.K., Oyasu, K., Hashimoto, T., Osumi, T., Rao, M.S., and Reddy, J.K. (1988)Cancer Res. 48, 5316–5324.

    PubMed  CAS  Google Scholar 

  33. Nicholis, P., and Schonbaum, G.R. (1963) inThe Enzymes 8 (Boyer, P.D., Lardy, H., and Myrback, K., eds) pp. 147–225, Academic Press, New York.

    Google Scholar 

  34. Hashimoto, F., and Hayashi, H. (1987)Biochim. Biophys. Acta 921, 142–150.

    PubMed  CAS  Google Scholar 

  35. Lazarow, P.B. (1982) inMetabolic Compartmentation (Sies, H., ed) pp. 317–329, Academic Press, New York.

    Google Scholar 

  36. Metzler, D.E. (1977) inBiochemistry—The Chemical Reaction of Living Cells, p. 474, Academic Press, New York.

    Google Scholar 

  37. Alexson, S.E.H., Fujiki, Y., Shio, H., and Lazarow, P.B. (1985)J. Cell. Biol. 101, 294–305.

    Article  PubMed  CAS  Google Scholar 

  38. Inestrosa, N.C., Bronfman, M., and Leighton, F. (1979)Life Sci. 25, 1127–1136.

    Article  PubMed  CAS  Google Scholar 

  39. Norseth, J., and Thomassen, M.S. (1983)Biochim. Biophys. Acta 751, 312–320.

    PubMed  CAS  Google Scholar 

  40. Bergseth, S., Christiansen, E.N., and Bremer, J. (1986)Lipids 21, 508–514.

    Article  PubMed  CAS  Google Scholar 

  41. Thomas, G., Loriette, C., Pepin, D., Chambaz, J., and Bereziat, G. (1988)Biochem. J. 256, 641–647.

    PubMed  CAS  Google Scholar 

  42. Christiansen, R.Z., Osmundsen, H., Borrebaek, B., and Bremer, J. (1978)Lipids 13, 487–491.

    Article  PubMed  CAS  Google Scholar 

  43. Christiansen, R.Z. (1978)Biochim. Biophys. Acta 530, 314–324.

    PubMed  CAS  Google Scholar 

  44. Singh, I., Moser, A.E., Goldfischer, S., and Moser, H.W. (1984)Proc. Natl. Acad. Sci. USA 81, 4203–4207.

    Article  PubMed  CAS  Google Scholar 

  45. Singh, H., Derwas, N., and Poulos, A. (1987)Arch. Biochem. Biophys. 259, 382–390.

    Article  PubMed  CAS  Google Scholar 

  46. Veerkamp, J.H., van Moerkerk, H.T.B., Glatz, J.F.C., and van Hinsbergh, V.W.M. (1983)Biochim. Biophys. Acta 753, 399–410.

    PubMed  CAS  Google Scholar 

  47. Chatzidakis, C., and Otto, D.A. (1987)Lipids 22, 620–626.

    PubMed  CAS  Google Scholar 

  48. Lazarow, P.B. (1978)J. Biol. Chem. 258, 1522–1528.

    Google Scholar 

  49. Osmundsen, H., and Bjornstad, K. (1985)Biochem. J. 230, 329–337.

    PubMed  CAS  Google Scholar 

  50. Laposata, M., Reich, E.L., and Majerus, P.W. (1985)J. Biol. Chem. 260, 11016–11020.

    PubMed  CAS  Google Scholar 

  51. Neufeld, E.J., Sprecher, H., Evans, R.W., and Majerus, P.W. (1984)J. Lipids Res. 25, 288–293.

    CAS  Google Scholar 

  52. Schepers, L., Casteels, M., Vamecq, J., Parmentier, G., van Veldhoven, P.P., and Mannaerts, G.P. (1988)J. Biol. Chem. 263, 2724–2731.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    F. A. G. Reubsaet, J. H. Veerkamp, J. M. F. Trijbels & L. A. H. Monnens

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  1. F. A. G. Reubsaet
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  2. J. H. Veerkamp
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  3. J. M. F. Trijbels
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  4. L. A. H. Monnens
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Reubsaet, F.A.G., Veerkamp, J.H., Trijbels, J.M.F. et al. Total and peroxisomal oxidation of various saturated and unsaturated fatty acids in rat liver, heart and m. quadriceps. Lipids 24, 945–950 (1989). https://doi.org/10.1007/BF02544539

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  • DOI: https://doi.org/10.1007/BF02544539

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