Abstract
Similarities in substrate specificity, localization and molecular weight between villus membrane phospholipase A2/lysophospholipase and carboxylester lipase of pancreatic origin suggested their possible identity. To test this, a preparation of the phospholipase A2/lysophospholipase released from brush border vesicles by papain was compared to authentic, pancreatic carboxylester lipase. Susceptibility of both activities to the inhibitor, diisopropylfluorophosphate, was consistent with their identity, but inconclusive. It also indicated that two populations of phospholipase A2 species may be present in the papain-released preparation. However, comparison of binding of the activities to Sepharose-coupled, anti-carboxylester-lipase IgG indicates that they are immunologically distinct.
Similar content being viewed by others
References
Pind, S., and Kuksis, A. (1987)Biochim. Biophys. Acta 901, 78–87.
Diagne, A., Mitiavila, S., Fauvel, J., Chap, H., and Douste-Blazy, L. (1987)Lipids 22, 33–40.
Gassama-Diagne, A., Fauvel, J., and Chap, H. (1989)J. Biol. Chem. 264, 9470–9475.
Pind, S., and Kuksis, A. (1988)Biochim. Biophys. Acta 938, 211–221.
Pind, S., and Kuksis, A. (1989)Lipids 24, 357–362.
Rudd, E.A., and Brockman, H.L. (1984) inLipases (Borgström, B., and Brockman, H.L., eds.) pp. 185–204, Elsevier Science Publishers, Amsterdam.
Kissel, J.A., Fontaine, R.N., Turck, C.W., Brockman, H.L., and Hui, D.Y. (1989)Biochim. Biophys. Acta 1006, 227–236.
Camulli, E.D., Linke, M.J., Brockman, H.L., and Hui, D.Y. (1989)Biochim. Biophys. Acta 1005, 177–182.
Han, J.H., Stratowa, C., and Rutter, W.J. (1987)Biochemistry 26, 1617–1625.
Lombardo, D., Fauvel, J., and Guy, O. (1980)Biochim. Biophys. Acta 611, 136–146.
David, J.S.K., Malathi, P., and Ganguly, J. (1966)Biochem. J. 98, 662–668.
Gallo, L.L., Chiang, Y., Vahouny, G.V., and Treadwell, C.R. (1980)J. Lipid Res. 21, 537–545.
Bosner, M.S., Gulick, T., Riley, D.J.S., Spilburg, C.A., and Lange, L.G., III (1988)Proc. Natl. Acad. Sci. USA 85, 7438–7442.
Rudd, E.A., Mizuno, N.K., and Brockman, H.L. (1987)Biochim. Biophys. Acta 918, 106–114.
Tsujita, T., and Brockman, H.L. (1987)Biochemistry 26, 8423–8429.
Bradford, M.M. (1976)Anal. Biochem. 72, 248–254.
Stout, J.S., Sutton, L.D., and Quinn, D.M. (1985)Biochim. Biophys. Acta 837, 6–12.
Subbaiah, P.V., and Ganguly, J. (1970)Biochem. J. 118, 233–239.
Author information
Authors and Affiliations
About this article
Cite this article
Mizuno, N.K., Brockman, H.L. Is intestinal villus phospholipase A2/lysophospholipase bound pancreatic carboxylester lipase?. Lipids 25, 760–762 (1990). https://doi.org/10.1007/BF02544048
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02544048