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Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector

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Lipids

Abstract

This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC) using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus assay. The present method was applied to rat heart and rat liver phospholipid analysis.

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Abbreviations

DPG:

diphosphatidylglycerol

HPLC:

high-performance liquid chromatography

LPC:

lysophosphatidylcholine

LPE:

lysophosphatidylethanolamine

LSD:

light-scattering detector

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

PS:

phosphatidylserine

SD:

standard deviation

SM:

sphingomyelin

UV:

ultraviolet

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Authors and Affiliations

  1. Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, 21034, Dijon cedex, France

    P. Juanéda, G. Rocquelin & P. O. Astorg

Authors
  1. P. Juanéda
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  2. G. Rocquelin
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  3. P. O. Astorg
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Juanéda, P., Rocquelin, G. & Astorg, P.O. Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector. Lipids 25, 756–759 (1990). https://doi.org/10.1007/BF02544047

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  • DOI: https://doi.org/10.1007/BF02544047

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