Skip to main content
SpringerLink
Log in

Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies

  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The lipid-free residue of lipid body membranes was isolated from quiescent peanuts and was physicochemically characterized. The preponderant component of the residue was proteinaceous and consisted of at least two polypeptides according to ultracentrifugation, gel filtration, gel electrophoresis and HPLC. The molecular weight of the principal polypeptide was between 10,000 and 16,000 daltons. Only one antigen, immunochemically unique with respect to other peanut components, was detected. Spectral analyses indicated the presence of a protoheme and revealed conformational modes of β-sheet and unordered structure but no α-helix. The amino acid composition was similar to that of an integral membrane polypeptide rather than to those of peripheral membranes or other plant polypeptides. The hydrophobicity, conformation and quantitative content of polypeptides were suitable for the existence of a monolayer at the lipid body-cytoplasm interface. The results indicated that lipid body coatings physicochemically resemble membranes of intracellular organelles and supported the morphological concept that the coatings are half-unit biological membranes. Reutilization of lipid body membranes appeared possible after lipid depletion during seed germination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Huang, A.H.C., inModern Methods of Plant Analysis, edited by H.F. Linskens and J.F. Jackson, Springer-Verlag, Berlin, Germany (1985) pp. 145–151.

    Google Scholar 

  2. Stymme, S., and A.K. Stobart, inThe Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, Academic Press, Orlando, Florida, (1987), pp. 175–214.

    Google Scholar 

  3. Yatsu, L.Y., and T.J. Jacks,Plant Physiol. 49:937 (1972).

    CAS  Google Scholar 

  4. Jacks, T.J., L.Y. Yatsu and A.M. Altschul,Plant Physiol. 42:585 (1967).

    CAS  Google Scholar 

  5. Minari, O., and D.B. Zilversmit,Anal. Biochem. 6:320 (1963).

    Article  CAS  Google Scholar 

  6. Jacks, T.J., L.Y. Yatsu and T.P. Hensarling,J. Am. Oil Chem. Soc. 47:222 (1970).

    CAS  Google Scholar 

  7. Soignet, D.M., R.J. Berni and R.R. Benerito,J. Appl. Polym. Sci. 20:2483 (1976).

    Article  CAS  Google Scholar 

  8. Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith,Anal. Chem., 28:350 (1956).

    Article  CAS  Google Scholar 

  9. Johansen, P.G., R.D. Marshall and A. Neuberger,Biochem. J. 77:239 (1960).

    CAS  Google Scholar 

  10. Lee, Y.C., and R. Montgomery,Arch. Biochem. Biophys. 93:292 (1961).

    Article  CAS  Google Scholar 

  11. Warren, L.,J. Biol. Chem. 234:1971 (1959).

    CAS  Google Scholar 

  12. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,J. Biol. Chem. 193:265 (1951).

    CAS  Google Scholar 

  13. Caldwell, I.C., and J.F. Henderson,Anal. Biochem. 34:303 (1970).

    Article  CAS  Google Scholar 

  14. LePecq, J.B., and C. Paoletti,Anal. Biochem., 17:100 (1966).

    Article  CAS  Google Scholar 

  15. Anson, M.L.,J. Gen. Physiol. 22:79 (1938).

    Article  CAS  Google Scholar 

  16. Salomom, L.L., J. James and P.R. Weaver,Anal. Chem. 36:1162 (1964).

    Article  Google Scholar 

  17. Nordlie, R.C., and W.A. Arion,Meth. Enzymol. 9:619 (1966).

    CAS  Google Scholar 

  18. Chantrenne, H.,Biochim. Biophys. Acta 16:410 (1955).

    Article  CAS  Google Scholar 

  19. Syrett, P.J., and P.C.L. John,151:295 (1968).

    CAS  Google Scholar 

  20. Racker, E.,J. Biol. Chem. 184:313 (1950).

    CAS  Google Scholar 

  21. Kornberg, A., and W.E. Pricer,J. Biol. Chem. 189:123 (1951).

    CAS  Google Scholar 

  22. Jacks, T.J., and H.W. Kircher,Anal. Biochem. 21:279 (1967).

    Article  CAS  Google Scholar 

  23. Singh, J., and A.R. Wasserman,Biochim. Biophys. Acta 221:379 (1970).

    CAS  Google Scholar 

  24. Greenfield, N., and G.D. Fasman,Biochemistry, 8:4108 (1969).

    Article  CAS  Google Scholar 

  25. Halsall, H.B.,Nature, 215:880 (1967).

    Article  CAS  Google Scholar 

  26. Schachman, H.K.,Meth. Enzymol. 4:32 (1957).

    Google Scholar 

  27. Laemmli, U.K.,Nature, 227:680 (1970).

    Article  CAS  Google Scholar 

  28. Shannon, C.F., and J.M. Hill,Biochemistry, 10:3021 (1971).

    Article  CAS  Google Scholar 

  29. Ouchterlony, O.,Acta Pathol. Microbiol. Scand. 26:507 (1949).

    CAS  Google Scholar 

  30. Grabar, P., and C.A. Williams,Biochim. Biophys. Acta, 10:193 (1953).

    Article  CAS  Google Scholar 

  31. Bjerrum, O.J., inMembrane Proteins: A Laboratory Manual, edited by A. Azzi, U. Brodbeck and P. Zahler, Springer-Verlag, Berlin, Germany, (1981) pp. 13–42.

    Google Scholar 

  32. Daussant, J., N.J. Neucere and L.Y. Yatsu,Plant Physiol. 44:471 (1969).

    CAS  Google Scholar 

  33. Neucere, N.J.,Anal. Biochem., 27:15 (1969).

    Article  CAS  Google Scholar 

  34. Yatsu, L.Y., T.J. Jacks and T.P. Hensarling,Plant Physiol. 48:675 (1971).

    CAS  Google Scholar 

  35. Altshul, A.M., L.Y. Yatsu, R.L. Ory and E.M. Engleman,Ann. Rev. Plant Physiol. 17:113 (1966).

    Article  Google Scholar 

  36. Yatsu, L.Y., and T.J. Jacks,Arch. Biochem. Biophys., 124:466 (1968).

    Article  CAS  Google Scholar 

  37. Tolbert, N.E.,Meth. Enzymol. 31:734 (1974).

    CAS  Google Scholar 

  38. Beevers, H., and R.W. Breidenbach,31:565 (1974).

    CAS  Google Scholar 

  39. Wiskich, J.T.,Biochem. Plants, 2:243 (1980).

    CAS  Google Scholar 

  40. Hensarling, T.P., L.Y. Yatsu and T.J. Jacks,J. Am. Oil Chem. Soc., 47:224 (1970).

    CAS  Google Scholar 

  41. Martin, S.S., and H.B. Bosmann,Exp. Cell Res. 55:59 (1971).

    Article  Google Scholar 

  42. Butler, W.L., and J.E. Baker,Nature, 205:1319 (1965).

    Article  CAS  Google Scholar 

  43. Harris, C.E., R.D. Kobes, D.C. Teller and W.J. Rutter,Biochemistry 8:2442 (1969).

    Article  CAS  Google Scholar 

  44. Vance, V.B., and A.H.C. Huang,J. Biol. Chem. 262:11275 (1987).

    CAS  Google Scholar 

  45. Yang, S., and R.S. Criddle,Biochemistry, 9:3063 (1970).

    Article  CAS  Google Scholar 

  46. Ji, T.H., J.L. Hess and A.A. Benson,Biochim. Biophys. Acta 150:676 (1968).

    Article  CAS  Google Scholar 

  47. Sprott, G.D., K.M. Shaw and K.F. Jarrell,J. Biol. Chem. 258:4026 (1983).

    CAS  Google Scholar 

  48. Henriques, F., and R.B. Park,Biochim. Biophys. Acta 430:312 (1976).

    Article  CAS  Google Scholar 

  49. Qu, R., S. Wang, Y. Lin, V.B. Vance and A.H.C. Huang,Biochem. J., 235:57 (1986).

    CAS  Google Scholar 

  50. Mani, R.S., and S. Zalik,Biochim. Biophys. Acta 200:132 (1970).

    CAS  Google Scholar 

  51. Holowach, L.P., J.F. Thompson and J.T. Madison,Plant Physiol. 74:576 (1984).

    CAS  Google Scholar 

  52. Neucere, N.J., and E.J. Conkerton,J. Agric. Food Chem. 26:683 (1978).

    Article  CAS  Google Scholar 

  53. Youle, R.J., and A.H.C. Huang,Amer. J. Bot. 68:44 (1981).

    Article  CAS  Google Scholar 

  54. Dechary, J.M., G.L. Leonard and S. Corkern,Lloydia, 33:270 (1970).

    CAS  Google Scholar 

  55. Jacks, T.J., T.P. Hensarling and L.Y. Yatsu,Econ. Bot. 26:135 (1972).

    CAS  Google Scholar 

  56. Cerff, R., and S.E. Chambers,J. Biol. Chem. 254:6094 (1979).

    CAS  Google Scholar 

  57. Bigelow, C.C.,J. Theoret. Biol. 16:187 (1967).

    Article  CAS  Google Scholar 

  58. Barrantes, F.J.,Biochem. Biophys. Res. Commun. 62:407 (1975).

    Article  CAS  Google Scholar 

  59. Miyazawa, T., and E.R. Blout,J. Am. Chem. Soc., 83:712 (1961).

    Article  CAS  Google Scholar 

  60. Miyazawa, T., Y. Masuda and K. Fukushima,J. Polymer Sci.: S62 (1962).

  61. Hoffpauir, C.L.,Agr. Food Chem., 1:668 (1953).

    Article  CAS  Google Scholar 

  62. Lin, Y.H., and A.H.C. Huang,Arch. Biochem. Biophys. 225:360 (1983).

    Article  CAS  Google Scholar 

  63. Ory, R.L., L.Y. Yatsu and H.W. Kircher,Arch. Biochem. Biophys. 264:225 (1968).

    Google Scholar 

  64. Simpson, T.D., and L.K. Nakamura,J. Am. Oil. Chem. Soc. 66:1093 (1989).

    CAS  Google Scholar 

  65. Huang, A.H.C., and R.A. Moreau,Planta, 141:111 (1978).

    Article  CAS  Google Scholar 

  66. Fernandez, D.E., R. Qu, A.H.C. Huang and L.A. Staehelin,Plant Physiol. 86:270 (1988).

    CAS  Google Scholar 

  67. Moreau, R.A., K.D.F. Liu and A.H.C. Huang,Plant Physiol. 65:1176 (1980).

    Article  CAS  Google Scholar 

  68. Slack, C.R., W.S. Bertaud, B.D. Shaw, R. Holland, J. Browse and H. Wright,Biochem. J. 190:551 (1980).

    CAS  Google Scholar 

  69. Cheeseman, D.F., and J.T. Davies,Advan. Protein Chem. 9:439 (1954).

    Article  Google Scholar 

  70. Fisher, H.F.,Biochim. Biophys. Acta 109:544 (1965).

    CAS  Google Scholar 

  71. Wanner, G., E.L. Vigil and R.R. Theimer,Planta, 156:314 (1982).

    Article  CAS  Google Scholar 

  72. Goldberg, D.B., S. Al-Marayati and E. Gonzalez,Plant Physiol. 68:280 (1982).

    Google Scholar 

  73. Vance, V.B., and A.H.C. Huang,J. Biol. Chem. 263:1476 (1988).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA

    T. J. Jacks, T. P. Hensarling, J. N. Neucere & L. Y. Yatsu

  2. American Fiber Manufacturers Association, 20036, Washington, DC

    R. H. Barker

Authors
  1. T. J. Jacks
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. P. Hensarling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. N. Neucere
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Y. Yatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. H. Barker
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Jacks, T.J., Hensarling, T.P., Neucere, J.N. et al. Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies. J Am Oil Chem Soc 67, 353–361 (1990). https://doi.org/10.1007/BF02539689

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02539689

Keywords

Search

Navigation