Abstract
To collect and exploitRoegneria genetic resources, isozyme variation on 7 different enzymes encoded by 26 presumptive loci were analyzed in leaf extracts ofR. kamoji, R. ciliaris, R. nakaii andTriticum aestivum cv. Chinese Spring (control species) using polyacrylamide gel electrophoresis. No isozyme polymorphism was detected within accession of the threeRoegneria species, all having self-pollinating reproduction. However, extensive variations at isozyme loci were observed among accessions ofR. kamoji — even among accessions distributed at different altitudes within the same collection area. These results suggested that: (1) a select few individuals collected at a collection site may be enough to represent the genetic variability of that population; and (2) collection and maintenance ofR. kamoji materials from different altitudes may be required to increase genetic diversity. Furthermore, the banded phenotypes at EST 1 and EST-2 loci may be used as a biochemical marker associated with a morphological character, pubescences covered at leaf margin inR. kamoji. The banded characters at EST- 1, SOD-1, SOD-2, and SOD-3a loci may be used as biochemical markers to identify theR. kamoji chromosomes carrying these loci in aT. aestivum ×R. kamoji hybridization program.
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Dong, Y. S., R. H. Zhou, S. J. Xu, L. H. Li, Y Cauderon & R.C. Wang, 1992. Desirable characteristics in perennial Triticeae collected in China for wheat improvement. Hereditas 116: 175–178
Hamrick, J. L. & M. J. Godt, 1990. Allozyme diversity in plant species. In: A. H. D. Brown, M. T. Clegg, A. L. Kahler & B. S. Weir (Eds), Plant population genetics, breeding, and genetic resources, pp. 43–63. Sinauer Associates Inc., Sunderland, Massachusetts, USA.
Jaaska, V., 1992. Isoenzyme variation in the grass genusElymus (Poaceae). Hereditas 117: 11–22.
Kuo, P. C., 1987. Flora Republicae Popularis Sinicae, Tomus 9(3), Science Press, Beijing, China.
Liu, D. J., 1986. The utilization of alien germplasm in wheat improvement. In: Z. S. Li & M. S. Swaminathan (Eds) Proc. 1st Int. Symp. Chromosome Engineering in Plants, pp. 176–178 Xi'an, China.
McIntyre, C. L., 1988. Variation at isozyme loci in Triticeae. Plant Syst. Evol. 160: 123–142.
Sanders, T. B., J. L. Hamrick & L. R. Holden, 1979. Allozyme variation inElymus canadensis from the tallgrass prairie region: Geographic variation. Am. Midl. Nat. 101: 1–12.
Tzvelev, N. N., 1983. Grasses of the Soviet Union. Oxonian Press, New Delhi.
Weeden, N. F. & J. F. Wendel, 1989. Genetics of plant isozymes. In: D. E. Soltis & P. S. Soltis (Eds), Isozymes in plant biology, pp. 46–72. Dioscorides Press, Portland, OR.
Wendel, J. F. & N. F. Weeden, 1989. Visualization and interpretation of plant isozymes. In: D. E. Soltis & P. S. Soltis (Eds), Isozymes in plant biology, pp. 5–45. Dioscorides Press, Portland, OR.
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Li, LH., Wange, R.R.C. & Dong, YS. Isozyme analysis of three species ofRoegneria C. Koch from China. Genet Resour Crop Evol 42, 119–125 (1995). https://doi.org/10.1007/BF02539515
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DOI: https://doi.org/10.1007/BF02539515