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Studies on the radiation inactivation of micro-organisms

IV. photoreactivation of the intermediate stages in the transformation ofBacillus cereus spores into vegetative cells

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Summary

The transformation ofBacillus cereus spores into vegetative cells has been studied. It was possible to distinguish between five different stages. Each stage could be isolated.

During the development of stage III cells from stage II spores the phenomenon of photoreactivation could be observed.

The results strongly suggest that the non-photoreactivability of UV-inactivated resting spores and stage II spores is due to their limited metabolic activity.

Résumé

Le développement des cellules végétatives des spores deBacillus cereus a été étudié. Il était possible de distinguer cinq états différents. Chaque état pouvait être isolé.

Le phénomène de la photoréactivation pouvait être observé pendant la transformation des spores de l’état II en de cellules de l’état III.

Les résultats indiquent nettement que l’absence de la photoréactivation des spores UV-inactivées de l’état I et de l’état II est due à leur activité métabolique limitée.

Zusammenfassung

Die Entwicklung von vegetativen Cellen aus Sporen vonBacillus cereus wurde untersucht. Es was möglich um fünf verschiedene Stadien zu unterscheiden. Jedes STadium konnte isoliert worden.

Während der Entwicklung von Stadium III Cellen aus Stadium II Sporen könnte die Photoreaktivierung beobachtet worden.

Die Resultaten deuten darauf, dass UV-inaktivierte Stadium I und Stadium II Sporen keine Photoreaktivierung zeigen weil ihre metabolische Aktivität zu beschränkt ist

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Part 1, 2 and 3: Biochim. biophys. Acta17, 206; in press.

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Stuy, J.H. Studies on the radiation inactivation of micro-organisms. Antonie van Leeuwenhoek 22, 337–349 (1956). https://doi.org/10.1007/BF02538347

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  • DOI: https://doi.org/10.1007/BF02538347

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