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Features of the Effect of VUV Radiation on Microfungi from Polar Regions

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Processes and Phenomena on the Boundary Between Biogenic and Abiogenic Nature

Part of the book series: Lecture Notes in Earth System Sciences ((LNESS))

Abstract

In this work, the effect of VUV radiation of the wavelength region Δλ = 166–180 nm on microscopic fungi propagules Cladosporium herbarum, Rhodotorula colostri, Saccharomyces cerevisiae was studied. In the course of the work, dependencies of the survival probability of propagules, which were at the exponential and stationary phases of development, on radiation doses were obtained. It was found that at the exponential stage of colonies development, the survival curves of propagules of different types of micromycetes coincide within the limits of error, but at the stationary phase they are different. IR spectroscopy and atomic force microscopy of irradiated propagules indicate a change in their cell wall. Electrophoresis of DNA molecules of irradiated propagules proves double-stranded breaks. Experiments with the use of an antioxidant show that the death of propagules during VUV irradiation occurs as a result of the direct and indirect effects of radiation, with the share of the latter being 10–15%. The results obtained allow us to conclude that the inactivation of propagules during irradiation with radiation of the long-wave region of the VUV range Δλ = 166–180 nm is the result of both direct and indirect effects with the destruction of both the cell wall and DNA.

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Acknowledgements

The authors want to express their gratitude to the E. Machs (Komarov Botanical Institute) for his help in carrying out DNA electrophoresis and to A. Vangonen (Vavilov State optical institute) for his help in recording the infrared transmission spectra of propagules.

This study was carried out as part of the state assignment according to the thematic plan of the Botanical Institute of the Russian Academy of Sciences (theme no. 01201255604) and the Basic Research Program of the Presidium of the Russian Academy of Sciences.

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Authors and Affiliations

  1. State University of Civil Aviation, 38 Pilotov Str., Saint Petersburg, 196210, Russia

    Galina N. Zvereva

  2. S. I. Vavilov State Optical Institute, Kadetskaya Line-5-2, Saint Petersburg, 199053, Russia

    Galina N. Zvereva

  3. Komarov Botanical Institute RAS, Professor Popov Str.-2, Saint Petersburg, 197376, Russia

    Irina Yu. Kirtsideli

Authors
  1. Galina N. Zvereva
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  2. Irina Yu. Kirtsideli
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Correspondence to Irina Yu. Kirtsideli .

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Editors and Affiliations

  1. Department of Crystallography, Saint Petersburg State University, Saint Petersburg, Russia

    Olga V. Frank-Kamenetskaya

  2. Department of Botany, Saint Petersburg State University, Saint Petersburg, Russia

    Dmitry Yu. Vlasov

  3. Department of Geochemistry, Saint Petersburg State University, Saint Petersburg, Russia

    Elena G. Panova

  4. Department of Physical Geography and Landscape Planning, Saint Petersburg State University, Saint Petersburg, Russia

    Sofia N. Lessovaia

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Zvereva, G.N., Kirtsideli, I.Y. (2020). Features of the Effect of VUV Radiation on Microfungi from Polar Regions. In: Frank-Kamenetskaya, O., Vlasov, D., Panova, E., Lessovaia, S. (eds) Processes and Phenomena on the Boundary Between Biogenic and Abiogenic Nature. Lecture Notes in Earth System Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-21614-6_32

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