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Analysis of triglycerides using atmospheric pressure chemical ionization mass spectrometry

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Lipids

Abstract

Atmospheric pressure chemical ionization (APCI) mass spectrometry was investigated as a new method for analysis of a mixture of triglycerides separated by reverse-phase high-performance liquid chromatography. A mixture of homogeneous (monoacid) triglyceride standards containing fatty acids with zero to three double bonds was analyzed to demonstrate the quality of mass spectra obtained by using the APCI interface. The mass spectra showed that minimal fragmentation occurs, resulting primarily in diglyceride [M−RCOO]+ ions and [M+1]+ protonated molecular ions. The degree of unsaturation within the acyl chains had a marked effect on the proportion of diglyceride ions vs. the [M+1]+ ions formed in the APCI source. The mass spectra of triglycerides containing fatty acids with two or three double bonds showed predominantly protonated triglyceride ions, with diglyceride peaks representing 13 to 25% of the base peak. The triglycerides containing singly unsaturated fatty acids gave diglyceride ions as the base peak, and [M+1]+ ions with an intensity 20 to 28% that of the base peak. Only diglyceride ions were observable in the spectra of triglycerides containing saturated fatty acids.

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Abbreviations

APCI:

atmospheric pressure chemical ionization

DG:

diglyceride

ELSD:

evaporative light-scattering detector

ESI:

electrospray ionization

HPLC:

high-performance liquid chromatography

MS:

mass spectrometry

RIC:

reconstructed ion chromatogram

RP-HPLC:

reverse-phase high-performance liquid chromatography

TG:

triglyceride

References

  1. Kuksis, A., Marai, L., and Myher, J.J. (1983)J. Chromatogr. 273, 43–66.

    PubMed  CAS  Google Scholar 

  2. Myher, J.J., Kuksis, A., Marai, L., and Manganaro, F. (1984)J. Chromatogr. 283, 289–301.

    Article  PubMed  CAS  Google Scholar 

  3. Kuksis, A., Myher, J.J., and Marai, L. (1985)J. Am. Oil Chem. Soc. 62, 762–767.

    Article  CAS  Google Scholar 

  4. Kuksis, A., and Myher, J.J. (1986)J. Chromatogr. 379, 57–90.

    PubMed  CAS  Google Scholar 

  5. Kallio, H., Laakso, P., Huopalahti, R., Linko, R.R., and Olsman, P. (1989)Anal. Chem. 61, 698–700.

    Article  PubMed  CAS  Google Scholar 

  6. Kuksis, A., Marai, L., and Myher, J.J. (1991)Lipids 26, 240–246.

    PubMed  CAS  Google Scholar 

  7. Merritt Jr., C., Vajdi, M., Kayser, S.G., Halliday, J.W., and Bazinet, M.L. (1982)J. Am. Oil Chem. Soc. 59, 422–432.

    CAS  Google Scholar 

  8. Rezanka, T., Husek, P., and Podojil, M. (1986)J. Chromatogr. 355, 265–271.

    Article  CAS  Google Scholar 

  9. Singleton, J.A., and Pattee, H.E. (1987)J. Am. Oil Chem. Soc. 64, 534–538.

    CAS  Google Scholar 

  10. Barber, M., Merren, T.O., and Kelly, W. (1964)Tetrahedron Lett., 1063–1067.

  11. Hites, R.A. (1970)Anal. Chem. 42, 1736–1740.

    Article  CAS  Google Scholar 

  12. Aasen, A.J., Lauer, W.M., and Holman, R.T. (1973)Lipids 5, 869–877.

    Article  Google Scholar 

  13. Lauer, W.M., Aasen, A.J., Graff, G., and Holman, R.T. (1973)Lipids 5, 861–868.

    Article  Google Scholar 

  14. Murata, T., and Takahashi, S. (1977)Anal. Chem. 49, 728–731.

    Article  PubMed  CAS  Google Scholar 

  15. Lehmann, W.D., and Kessler, M. (1983)Biomed. Mass Spectrom. 10, 220–226.

    Article  PubMed  CAS  Google Scholar 

  16. Barber, M., Tetler, L.W., Bell, D., Ashcroft, A.E., Brown, R.S., and Moore, C. (1987)Org. Mass Spectrom. 22, 647–650.

    Article  CAS  Google Scholar 

  17. Showell, J.S., Fales, H.M., and Sokoloski, E.A. (1989)Org. Mass Spectrom. 24, 632–636.

    Article  CAS  Google Scholar 

  18. Duffin, K.L., Henion, J.D., and Shieh, J.J. (1991)Anal. Chem. 63, 1781–1788.

    Article  PubMed  CAS  Google Scholar 

  19. Mares, P., Rezanka, T., and Novak, M. (1991)J. Chromatogr. 568, 1–10.

    PubMed  CAS  Google Scholar 

  20. Rezanka, T., and Mares, P. (1991)J. Chromatogr. 542, 145–159.

    Article  CAS  Google Scholar 

  21. Covey, T.R., Lee, E.D., Bruins, A.P., and Henion, J.D. (1986)Anal. Chem. 58, 1451A-1461A.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. FQS, NCAUR, ARS, USDA, 1815 N. University St., 61604, Peoria, Illinois

    Wm. Craig Byrdwell & Edward A. Emken

Authors
  1. Wm. Craig Byrdwell
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  2. Edward A. Emken
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Byrdwell, W.C., Emken, E.A. Analysis of triglycerides using atmospheric pressure chemical ionization mass spectrometry. Lipids 30, 173–175 (1995). https://doi.org/10.1007/BF02538272

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  • DOI: https://doi.org/10.1007/BF02538272

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