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Fatty Acid Composition by Total Acyl Lipid Collision-Induced Dissociation Time-of-Flight (TAL-CID-TOF) Mass Spectrometry

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Plant Lipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2295))

Abstract

Total acyl lipid collision-induced dissociation time-of-flight (TAL-CID-TOF) mass spectrometry uses a quadrupole time-of-flight (QTOF) mass spectrometer to rapidly provide a comprehensive fatty acid composition of a biological lipid extract. Samples are infused into a QTOF instrument, operated in negative mode, and the quadrupole is used to transfer all, or a wide mass range of, precursor ions to the collision cell for fragmentation. Time-of-flight-acquired mass spectra provide mass accuracy and resolution sufficient for chemical formula determination of fatty acids in the complex mixture. Considering the limited number of reasonable CHO variants in fatty acids, one can discern acyl anions with the same nominal mass but different chemical formulas. An online application, LipidomeDB Data Calculation Environment, is employed to process the mass spectral output data and match identified fragments to target fragments at a resolution specified by the user. TAL-CID-TOF methodology is a useful discovery or screening tool to identify and compare fatty acid profiles of biological samples.

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Acknowledgments

The authors would like to thank Mary Roth for sharing her expertise in sample preparation methods. This work was supported by the USDA National Institute of Food and Agriculture, Hatch/Multi-State project 1013013, National Science Foundation MCB 1413036, and the Chemical Biology of Infectious Disease, Center of Biomedical Research Excellence (COBRE) of National Institute of Health (P20GM113117). Instrument acquisition and maintenance at KLRC was supported by National Science Foundation (EPS 0236913, DBI 0521587, DBI 1228622, DBI 1726527), K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20GM103418), and Kansas State University. Contribution no. 20-230-B from the Kansas Agricultural Experiment Station.

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Authors and Affiliations

  1. Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS, USA

    Pamela Tamura, Carl Fruehan & Ruth Welti

  2. Computational Chemical Biology Core, University of Kansas, Lawrence, KS, USA

    David K. Johnson

  3. Waters Corporation, Milford, MA, USA

    Paul Hinkes

  4. Mass Spectrometry Laboratory, University of Kansas, Lawrence, KS, USA

    Todd D. Williams

Authors
  1. Pamela Tamura
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  2. Carl Fruehan
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  3. David K. Johnson
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  4. Paul Hinkes
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  5. Todd D. Williams
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  6. Ruth Welti
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Corresponding author

Correspondence to Ruth Welti .

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Editors and Affiliations

  1. Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Bonn, Germany

    Dorothea Bartels

  2. IMBIO Institut, Universität Bonn, Bonn, Nordrhein-Westfalen, Germany

    Peter Dörmann

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Tamura, P., Fruehan, C., Johnson, D.K., Hinkes, P., Williams, T.D., Welti, R. (2021). Fatty Acid Composition by Total Acyl Lipid Collision-Induced Dissociation Time-of-Flight (TAL-CID-TOF) Mass Spectrometry. In: Bartels, D., Dörmann, P. (eds) Plant Lipids. Methods in Molecular Biology, vol 2295. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1362-7_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1362-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1361-0

  • Online ISBN: 978-1-0716-1362-7

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