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Measurement of lipid peroxidation in vivo: A comparison of different procedures

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Lipids

Abstract

A study was undertaken to investigate whether some of the methods commonly used to detect lipid peroxidation of cellular membranes in vivo correlate with each other. The study was performed with the livers of bromobenzene-intoxicated mice, in which lipid peroxidation develops when the depletion of glutathione (GSH) reaches a threshold value. The methods tested and compared were the following: i) measurement of the malondialdehyde (MDA) content of the liver; ii) detection of diene conjugation absorption in liver phospholipids; iii) measurement of the loss of polyunsaturated fatty acids in liver phospholipids; and iv) determination of carbonyl functions formed in acyl residues of membrane phospholipids as a result of the peroxidative breakdown of phospholipid fatty acids. Correlations among the values obtained with these methods showed high statistical significances, indicating that the procedures measure lipid peroxidation in vivo with comparable reliability. Analogously, the four methods appeared also to correlate when applied to in vitro microsomal lipid peroxidation.

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Authors and Affiliations

  1. Istituto di Patologia Generale dell'Università di Siena, Via Laterino 8, 53100, Siena, Italy

    Alfonso Pompella, Emilia Maellaro, Alessandro F. Casini, Marco Ferrali, Lucia Ciccoli & Mario Comporti

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  1. Alfonso Pompella
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  2. Emilia Maellaro
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  3. Alessandro F. Casini
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  4. Marco Ferrali
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  5. Lucia Ciccoli
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  6. Mario Comporti
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Pompella, A., Maellaro, E., Casini, A.F. et al. Measurement of lipid peroxidation in vivo: A comparison of different procedures. Lipids 22, 206–211 (1987). https://doi.org/10.1007/BF02537304

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