Abstract
Mice fed menhaden (fish) oil or coconut oil-rich diets were inoculated intraperitoneally with a rapidly growing leukemia, T27A. After one week, the tumor cells were harvested, and51Cr was used to label intracellular molecules. Spontaneous release of51Cr was used as a measure of plasma membrane permeability. Compared to cells from mice fed coconut oil (rich in saturated fatty acids), tumor cells from mice fed menhaden oil (rich in long chain polyunsaturated ω3 fatty acids) showed an increased level of spontaneous51Cr release, which was exacerbated by increased temperature and reduced by extracellular protein. At physiological salt concentrations, the releated51Cr was detected in particles of ∼2700 daltons. Enhanced permeability correlated with the incorporation of dietary (fish oil) ω3 polyunsaturated fatty acids docosahexaenoic and eicosapentaenoic acid into the tumor cells. The results demonstrate that ω3 fatty acids are incorporated into cellular constituents of tumor cells and change properties associated with the plasma membrane. This result suggests that dietary manipulation may be used to enhance tumor cell permeability and contribute to tumor eradication.
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Abbreviations
- ANOVA:
-
analysis of variance
- BSA:
-
bovine serum albumin
- CCCP:
-
carbonyl cyanidem-chlorophenylhydrazone
- DHA:
-
docosahexaenoic acid
- 2,4-DNP:
-
2,4-dinitrophenol
- EPA:
-
eicosapentaenoic acid
- FBS:
-
fetal bovine serum
- PUFA:
-
polyunsaturated fatty acid
- RBC:
-
red blood cell
- SFA:
-
saturated fatty acid
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Jenski, L.J., Sturdevant, L.K., Ehringer, W.D. et al. ω3 Fatty acids increase spontaneous release of cytosolic components from tumor cells. Lipids 26, 353–358 (1991). https://doi.org/10.1007/BF02537198
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DOI: https://doi.org/10.1007/BF02537198