Abstract
The protective effects of vitamin E (α-tocopherol) and its model compounds, which act as antioxidants, on linoleic acid hydroperoxide-induced injury to human umbilical vein endothelial cells were examined. When incubated at 50 μM with endothelial cells at 37°C for 24 hr, α-tocopherol protected the cells from injury, and 2,2,5,7,8-pentamethylchroman-6-ol showed a similar protective effect. Trolox C, a water-soluble vitamin E model compound, had no protective effect. Tocol, a poor antioxidant, proved toxic. During preincubation, α-tocopherol was incorporated into the cells at 16.6 nmol/ mg protein, while the pentamethylchromanol was incorporated at 0.5 nmol/mg protein; Trolox C was not incorporated at all. The results suggest that agents having both high antioxidant activity and lipophilicity can protect endothelial cells from linoleic acid hydroperoxide-induced injury.
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Abbreviations
- ECGF:
-
endothelial cell growth factor
- EGF:
-
epidermal growth factor
- FBS:
-
fetal bovine serum
- HPLC:
-
high-performance liquid chromatography
- LOOH:
-
linoleic acid hydro-peroxide
- PBS:
-
phosphate buffered saline
- PMC:
-
2,2,5,7,8-pentamethylchroman-6-ol
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Issued as TMIG-I No. 170.
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Kaneko, T., Nakano, Si. & Matsuo, M. Protective effect of vitamin E on linoleic acid hydroperoxide-induced injury to human endothelial cells. Lipids 26, 345–348 (1991). https://doi.org/10.1007/BF02537196
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DOI: https://doi.org/10.1007/BF02537196