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A rapid method for the preparation of ganglioside Glac2 (GD3)

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Lipids

Abstract

A method is described for the preparation of ganglioside Glac2 [(II3(NeuAc)2-LacCer, GD3] from cream of bovine milk using liquid-phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14–15 L of bovine milk, yields approximately 70 mg of pure ganglioside Glac2. The sialic acid constituent of ganglioside Glac2 isolated from bovine milk cream consists solely of theN-acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing-4-enine and the 22∶0 (behenic acid) and 23∶0 fatty acids.

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Abbreviations

DEAE:

diethylaminoethyl

I/H/W:

isopropanol/n-hexane/water

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Physiologisch-Chemisches Institut, Philipps-Universität, 3550, Marburg, Germany

    Richard Jennemann & Herbert Wiegandt

Authors
  1. Richard Jennemann
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  2. Herbert Wiegandt
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Additional information

Short hand notations for gangliosides are according to Wiegandt (Ref. 1). Glac2, (II3(NeuAc)2-LacCer) is NeuAcα,8NeuAcα,-3Galβ,4Glcβ,1Cer; Gtet1, (II3NeuAc-Gg4Cer) is Gal\,3GalNAcβ,-4(NeuAcα,3)Galβ,4Glcβ,1Cer.

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Jennemann, R., Wiegandt, H. A rapid method for the preparation of ganglioside Glac2 (GD3). Lipids 29, 365–368 (1994). https://doi.org/10.1007/BF02537191

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  • DOI: https://doi.org/10.1007/BF02537191

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