Abstract
The effects of the polyynsaturated fatty acids (PUFAs)—eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (AA)—onin vitro steroid production by full-grown prematurational ovarian follicles from goldfish and rainbow trout were investigated. EPA and DHA inhibited gonadotropin-stimulated testosterone production in a dose-related manner, but AA was inhibitory only at the highest dose tested (400 μM). AA alone stimulated testosterone production by increasing cAMP production, but the effects of other PUFAs alone were marginal. The inhibitory actions by PUFAs were not restricted to long-chain PUFAs, as linoleic and linolenic acids had similar actions in the goldfish. The inhibitory action of EPA on testosterone production was reversible upon removal of the PUFA from medium. Testosterone production stimulated by the addition of the cAMP analogues, dibutyryl cAMP, and 8-bromo cAMP, was attenuated by PUFAs, suggesting that they act at a site distal to cAMP formation. A post-cAMP site regulating cholesterol availability may be involved as testosterone production induced by addition of 25OH-cholesterol was not affected by the PUFAs in either fish species. Together, these findings underscore the importance of lipids in ovarian physiology and suggest that PUFAs may participate in the regulation of ovarian steroidogenesis in teleost fish.
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Abbreviations
- AA:
-
arachidonic acid
- 25-OH Chol:
-
25-hydroxycholesterol
- dbcAMP:
-
dibutyryl cAMP
- DHA:
-
docosahexaenoic acid
- DMSO:
-
dimethyl sulfoxide
- EPA:
-
eicosapentaenoic acid
- hCG:
-
human chorionic gonadotropin
- IBMX:
-
3-isobutyl-1-methylxanthine
- PKC:
-
protein kinase C
- PMA:
-
phorbol 12-myristate 13-acetate
- PUFA:
-
polyunsaturated fatty acid
- SG-G100:
-
partially purified salmon gonadotropin
References
Hazel, J.R. (1984)Am. J. Physiol. 246, R460-R470.
Bell, M.V., Henderson, R.J., and Sargent, J.R. (1986)Comp. Biochem. Physiol. 83B, 711–719.
Tocher, D.R., and Sargent, J.R. (1990)Lipids 25, 435–442.
Hazel, J.R., and Williams, E.E. (1990)Prog. Lipid Res. 29, 167–227.
Henderson, R.J., and Tocher, D.R. (1987)Prog. Lipid Res. 26, 281–347.
Downes, C.B., and Michell, R.H. (1985) inMolecular Mechanisms of Transmembrane Signalling (Cohen, P., and Houslay, M.D., eds.) pp. 3–56, Elsevier, New York.
Rasmussen, H., Apfeldorf, W., Barrett, P., Takuwa, N., Zawalich, W., Kreutter, D., Park, S., and Takuwa, Y. (1986) inPhosphoinositides and Receptor Mechanisms (Putney, J.W., ed.) pp. 109–148, Alan R. Liss Inc., New York.
Smith, W.L. (1989)Biochem. J. 259, 315–324.
Naor, Z. (1991)Mol. Cell. Endocrinol. 80, C181-C186.
Murdoch, W.J., Hansen, T.R., and McPherson, L.A. (1993)Prostaglandins 46, 85–115.
Simopoulos, A.P., Kifer, R.R., and Martin, R.E. (1986) inHealth Effects of Polyunsaturated Fatty Acids in Seafoods, Academic Press, New York.
Lees, R.S., and Karel, M. (1990)Omega-3 Fatty Acids in Health and Disease, Marcel Dekker, Inc., New York.
Phleger, C.F., Laub, R.J., and Benson, A.A. (1989)Lipids 24, 286–289.
Fremont, L., and Marion, D. (1982)Comp. Biochem Physiol. 73B, 849–856.
Watanabe, T., Takeuchi, T., Saito, M., and Nishimura, K. (1984)Bull. Japan. Soc. Sci. Fish. 50, 1207–1215.
Wiegand, M.D., and Idler, D.R. (1985)Can. J. Zool. 63, 2775–2777.
Wade, M.G., Van Der Kraak, G., Gerrits, M.F., and Ballantyne, J.S. (1994)Biol. Reprod. 51, 131–139.
Van Der Kraak, G., and Chang, J.P. (1990)Gen. Comp. Endocrinol. 77, 221–228.
Wade, M.G., and Van Der Kraak, G. (1993)Gen. Comp. Endocrinol. 90, 109–118.
Meikle, A.W., Benson, S.J., Liu, X.-H., Boam, W.D., and Stringham, J.D. (1989)Am. J. Physiol. 257, E937-E942.
Johnson, A.L., and Tilly, J.L. (1990)Biol. Reprod. 42, 458–464.
Johnson, A.L., and Tilly, J.L. (1990)Biol. Reprod. 43, 922–928.
Speizer, L.A., Watson, M.J., and Brunton, L.L. (1991)Am. J. Physiol. 261, E109-E114.
Lopez-Ruiz, M.P., Choi, M.S.K., Rose, M.P., West, A.P., and Cooke, B.A. (1992)Endocrinology 130, 1122–1130.
Elliott, M.E., and Goodfriend, T.L. (1993)Endocrinology 132, 2453–2460.
Kagawa, H., Young, G., and Nagahama, Y. (1984)Gen. Comp. Endocrinol. 54, 139–143.
Kime, D.E. (1993)Rev. Fish Biol. Fish. 3, 160–180.
Van Der Kraak, G. (1992)J. Exp. Zool. 262, 271–278.
Fontaine, Y.A., Burzawa-Gerard, E., and Delerue-Le Belle, N. (1970)C.R. Acad. Sci. Paris Ser. D271, 780–783.
Kanamori, A., and Nagahama, Y. (1988)Gen. Comp. Endocrinol. 72, 39–53.
Srivastava, R.K., and Van Der Kraak, G. (1994)Gen. Comp. Endocrinol. 95, 60–70.
Rennert, H., Amsterdam, A., Billheimer, J.T., and Strauss III, J.F. (1991)Biochemistry 30, 11280–11285.
Yamamoto, R., Kallen, C.B., Babalola, G.O., Rennert, H., Billheimer, J.T., and Strauss, III, J.F. (1991)Proc. Natl. Acad. Sci. USA 88, 463–467.
Sordoillet, C., Chauvin, M.A., Hendrick, J.C., Franchimont, P., Morera, A.M., and Benahmed, M. (1992)Endocrinology 130, 1352–1358.
Engelhardt, H., Tekpetey, F.R., Gore-Langton, R.E., and Armstrong, D.T. (1992)Mol. Cell. Endocrinol. 85, 117–126.
Mauduit, C., Chauvin, M.A., Hartmann, D.J., Revol, A., Morera, A.M., and Benahmed, M. (1992)Biol. Reprod. 46, 1119–1126.
Golos, T.G., Strauss III, J.F., and Miller, W.L. (1987)J. Steroid Biochem. 27, 767–773.
Nestler, J.E., Takagi, K., and Strauss III, J.F. (1990) inAdvances in Cholesterol Research (Esfahani, M., and Swaney, J.B., eds.) pp. 133–170, The Caldwell Press, New Jersey.
Billheimer, J.T., and Gillies, P.J. (1990) inAdvances in Cholesterol Research (Esfahani, M., and Swaney, J.B., eds.) pp. 7–46, The Caldwell Press, New Jersey.
Leung, P.C.K., and Steele, G.L. (1992)Endo. Rev. 13, 476–498.
Steele, G.L., and Leung, P.C.K. (1993) inThe Ovary (Adashi, E.Y., and Leung, P.C.K., eds.) pp. 113–127, Raven Press, Ltd., New York.
Ranjan, M., and Goetz, F.W. (1987)J. Exp. Zool. 242, 355–361.
Van Der Kraak, G. (1990)Biol. Reprod. 42, 231–238.
Van Der Kraak, G. (1991)Gen. Comp. Endocrinol. 81, 268–275.
Srivastava, R.K., and Van Der Kraak, G. (1994)Gen. Comp. Endocrinol. 93, 181–191.
Goetz, F.W. (1993)Biol. Reprod. 48, 846–850.
Nishizuka, Y. (1984)Nature 308, 693–697.
Berridge, M.J. (1984)Biochem. J. 220, 345–360.
Wade, M.G., and Van Der Kraak, G. (1995)Biol. Reprod. 51, 131–139.
Greene, D.H., and Selivonchick, D.P. (1987)Prog. Lipid Res. 26, 53–85.
Orton, T.C., and Parker, G.L. (1982)Drug. Metab. Dispos. 10, 110–115.
Reddy, J.K., Goel, S.K., Nemali, M.R., Carrino, J.J., Laffler, T.G., Reddy, M.K., Sperbeck, S.J., Osumi, T., Hashimoto, T., Lalwni, N.D., and Rao, M.S. (1986)Proc. Natl. Acad. Sci. USA 83, 1747–1751.
Issemann, I., and Green, S. (1990)Nature 347, 645–650.
Gottlicher, M., Widmark, E., Li, Q., and Gustafsson, J.-A. (1992)Proc. Natl. Acad. Sci. USA 89, 4653–4657.
Issemann, I., Prince, R.A., Tugwood, J.D., and Green, S. (1993)J. Mol. Endocrinol. 11, 37–47.
Keller, H., Dreyer, C., Medin, J., Mahfoudi, A., Ozato, K., and Wahli, W. (1993)Proc. Natl. Acad. Sci. USA 90, 2160–2164.
Lock, E.A., Mitchell, A.M., and Elcombe, C.R. (1989)Annu. Rev. Pharmacol. Toxicol. 29, 145–163.
Auwerx, J. (1992)Horm. Res. 38, 269–277.
Bell, D.R., and Elcombe, C.R. (1993) inPeroxisomes: Biology and Importance in Toxicology and Medicine (Gibson, G., and Lake, B., eds.) pp. 137–148, Taylor and Francis, Washington, D.C.
Ballantyne, J.S., Glemet, H.C., Chamberliin, M.E., and Singer, T.D. (1993)Mar. Biol. 116, 47–52.
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Mercure, F., Van Der Kraak, G. Inhibition of gonadotropin-stimulated ovarian steroid production by polyunsaturated fatty acids in teleost fish. Lipids 30, 547–554 (1995). https://doi.org/10.1007/BF02537030
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DOI: https://doi.org/10.1007/BF02537030