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Absorption of triglycerides with defined or random structure by rats with biliary and pancreatic diversion

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Lipids

Abstract

Fat absorption may be compromised by pancreatic or bile insufficiency, resulting in low uptake of essential fatty acid and energy. Using a rat model of malabsorption, we examined the absorption of defined triglycerides with medium-chain fatty acids (MCFA) in thesn-1,3 positions and essential fatty acids in thesn-2 position (MLM) compared to other fats. The thoracic duct was cannulated for collection of lymph, and the common bile and pancreatic duct was cannulated to divert both the pancreatic juice and bile. The rats were given a single bolus of triglyceride as a taurocholate emulsion. Fat absorption was measured from collected lymph samples. The triglycerides administered were a defined triglyceride, MLM [mainly (8∶0/10∶0)-(18∶2n−6)-(8∶0/10∶0)], a similar triglyceride subjected to chemical randomization, a mixture of medium-chain triglycerides and soybean oil, and soybean oil, respectively. The first three triglycerides had approximately 36 wt% linoleic acid (18∶2n−6) content. Administration of defined triglyceride was followed by significantly higher lymphatic level (wt%) of 18∶2n−6 (P<0.01) as well as a relative enhancement in mol% of 18∶2n−6 (P<0.05) compared to the other triglycerides. Lymphatic absorption of MCFA was similar in the three first groups but not as efficient as for long-chain fatty acids. Our results indicate that defined triglycerides thus may provide a means to increase absorption of essential fatty acids in fat malabsorption, such as that seen in cystic fibrosis, or for pre-term infants.

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Abbreviations

EFA:

essential fatty acids

GLC:

gas-liquid chromatography

LCFA:

long-chain fatty acids

MCFA:

medium-chain fatty acids

MCT:

medium-chain triglycerides

MLM:

defined triglycerides with medium-chain, long-chain, medium-chain fatty acids

TG:

triglyceride(s)

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark

    Michael Søberg Christensen & Carl-Erik Høy

  2. Center for Food Research, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark

    Michael Søberg Christensen & Carl-Erik Høy

  3. Novo Nordisk A/S, DK-2880, Bagsvaerd, Denmark

    Anette Müllertz

Authors
  1. Michael Søberg Christensen
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  2. Anette Müllertz
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  3. Carl-Erik Høy
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Honored Student presentation, 84th AOCS Annual Meeting & Expo, Anaheim, California.

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Christensen, M.S., Müllertz, A. & Høy, CE. Absorption of triglycerides with defined or random structure by rats with biliary and pancreatic diversion. Lipids 30, 521–526 (1995). https://doi.org/10.1007/BF02537026

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