Skip to main content
SpringerLink
Log in

Annexin I in female rabbit reproductive organs: Varying levels in relation to maturity and pregnancy

  • Article
  • Published:
Lipids

Abstract

The level of annexin I, a 36 kDa calcium-dependent phospholipid-binding protein (36 kDa PLBP) in the reproductive organs of young, mature, and pregnant rabbits was determined immunologically with antibodies raised against purified rabbit lung annexin I. In the cytosolic fractions of the ovary, fallopian tube, uterus, and placenta, annexin I was the only major immunoreactive protein. The reproductive organs appeared to have higher annexin I levels than most nonreproductive organ tissues, except the lung and the spleen which were also rich in annexin I. A small amount of annexin I and a nearly equal amount of its hydrolytic product, a 33 kDa polypeptide, were detected in the amniotic fluid between 21 and 27 days gestation. Structural similarity of annexin I in the reproductive organs and in the lung was suggested by their identical isoelectric point values. Annexin I in the ovary of adult rabbits was 70% higher than that in the respective organ of immature rabbits. The uterus of pregnant rabbits had about 84% higher annexin I contents than that of the nonpregnant rabbits., The placenta had more annexin I per mg cytosolic protein than either the ovary or the uterus during pregnancy. The high concentration of annexin I in the reproductive organs may reflect specific functions of these organs in the reproductive years and during the reproductive cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ELISA:

enzyme-linked immunosorbent assay

IEF:

isoelectric focusing

PLBP:

phospholipid-binding protein

References

  1. Romisch, J., and Paques, E.P. (1991) Annexins: Calcium-Binding Proteins of Multi-Functional Importance?Med. Microbiol. Immunol. 180, 109–126.

    Article  PubMed  CAS  Google Scholar 

  2. Creutz, C.E. (1992) The Annexins and Exocytosis,Science 258, 924–931.

    Article  PubMed  CAS  Google Scholar 

  3. Pollard, H.B., Burns, A.L., and Rojas, E. (1990) Synexin (annexin VII): A Cytosolic Calcium-Binding Protein Which Promotes Membrane Fusion and Forms Calcium Channels in Artificial Bilayer and Natural Membranes,J. Membr. Biol. 117, 101–112.

    Article  PubMed  CAS  Google Scholar 

  4. Flower, R. (1989) Lipocortin,Biochem. Soc. Trans. 17, 276–280.

    PubMed  CAS  Google Scholar 

  5. Russo-Marie, F. (1991) Lipocortins: An Update,Prostagland. Leuk. Essent. Fatty. 42, 83–89.

    Article  CAS  Google Scholar 

  6. Whitehouse, B.J. (1989) Lipocortins, Mediators of the Anti-Inflammatory Actions of Corticosteroids,J. Endocrinol. 123, 363–366.

    Article  PubMed  CAS  Google Scholar 

  7. Pepinsky, R.B., Tizard, R., Mattaliano, R.J., Sinclair, L.K., Miller, G.T., Browning, J.L., Chow, P., Burne, C., Huang K.S., Pratt, D., Wachter, L., Hession, C., Frey, A.Z., and Wallner, B.P. (1988) Five Distinct Calcium and Phospholipid Binding Proteins Share Homology with Lipocortin I,J. Biol. Chem. 263, 10799–10811.

    PubMed  CAS  Google Scholar 

  8. Melgunov, V.I. (1991) Annexins—A New Family of Ca2+-Binding Proteins,Biochemistry-USSR. 56, 204–224.

    CAS  Google Scholar 

  9. Hauptmann, R., Maurer-Fogy, I., Krystek, E., Bodo, G., Andree, H., and Reutelingsperger, C.P.M. (1989) Vascular Anticoagulant β: A Novel Human Ca2+/Phospholipid Binding Protein That Inhibits Coagulation and Phospholipase A2 Activity. Its Molecular Cloning, Expression and Comparison with VAC-α,Eur. J. Biochem. 185, 63–71.

    Article  PubMed  CAS  Google Scholar 

  10. Pepinsky, R.B., and Hauptmann, R. (1992) Detection of VAC-β (annexin-8) in Human Placenta,FEBS Lett. 306, 85–89.

    Article  PubMed  CAS  Google Scholar 

  11. Tsao, F.H.C., Chen, X.R., Chen, X.M., and Vu, V.X. (1994) Immunocharacterization and Developmental Regulation of Rabbit Lung Calcium-Dependent Phospholipid-Binding Proteins,Biochim. Biophys. Acta 1213, 91–99.

    PubMed  CAS  Google Scholar 

  12. Kaetzel, M.A., Hazarika, P., and Dedman, J.R. (1989) Differential Tissue Expression of Three 35-kDa Annexin Calcium-Dependent Phospholipid-Binding Proteins,J. Biol. Chem. 264, 14463–14470.

    PubMed  CAS  Google Scholar 

  13. Lynch-Salamon, D.I., Everson, W.V., and Myatt, L. (1992) Decrease in Annexin I Messenger Ribonucleic Acid Expression in Human Amnion with Labor,Am. J. Obstet. Gynecol. 167, 1657–1663.

    PubMed  CAS  Google Scholar 

  14. Myatt, L., Hirth, J., and Everson, W.V. (1992) Changes in Annexin (lipocortin) Content in Human Amnion and Chorion at Parturition,J. Cell. Biochem. 50, 363–373.

    Article  PubMed  CAS  Google Scholar 

  15. Tsao, F.H.C. (1990) Purification and Characterization of Two Rabbit Lung Ca2+-Dependent Phospholipid-Binding Proteins,Biochim. Biophys. Acta 1045, 29–39.

    PubMed  CAS  Google Scholar 

  16. Tsao, F.H.C., Hull, W.M., Strickland, M.S., Whitsett, J.A. Foo, T.K.F., Zografi, G., and DeLuca, P.M., Jr. (1991) Lung Calcium-Dependent Phospholipid-Binding Proteins: Structure and Function,Biochim. Biophys. Acta 1081, 141–150.

    PubMed  CAS  Google Scholar 

  17. Khanna, N.C., Helwig, E.D., Ikebuchi, W., Fitzpatrick, S., Bajwa, R., and Waisman, D.M. (1990) Purification and Characterization of Annexin Proteins from Bovine Lung,Biochemistry 29, 4852–4862.

    Article  PubMed  CAS  Google Scholar 

  18. Romisch, J., and Heimburger, N. (1990) Purification and Characterization of Six Annexins from Human Placenta,Biol. Chem. Hoppe-Seyler. 371, 383–388.

    PubMed  CAS  Google Scholar 

  19. Pepinsky, R.B., Sinclair, L.K., Browning, J.L., Mattaliano, R.J., Smart, J.E., Chow, E.P., Falbel, T., Ribolini, A., Garwin, J.L., and Wallner, B.P. (1986) Purification and Partial Sequence Analysis of a 37-kDa Protein That Inhibits Phospholipase A2 Activity from Rat Peritoneal Exudates,J. Biol. Chem. 261, 4239–4246.

    PubMed  CAS  Google Scholar 

  20. Christmas, P., Callaway, J., Fallon, J., Jones, J., and Haigler, H.T. (1991) Selective Secretion of Annexin 1, A Protein Without a Signal Sequence, by the Human Prostate Gland,J. Biol. Chem. 266, 2499–2507.

    PubMed  CAS  Google Scholar 

  21. Hook, G.E.R., Gilmore, L.B., Tombropoulos, E.G., and Fabro, S.E. (1978) Fetal Lung Lamellar Bodies in Human Amniotic Fluid,Am. Rev. Respir. Dis. 117, 541–550.

    PubMed  CAS  Google Scholar 

  22. Das, S.K., Chakrabarti, P., Tsao, F.H.C., Nayyar, T., and Mukherjee, S. (1992) Identification of Calcium-Dependent Phospholipid-Binding Proteins (annexins) from Guinea Pig Alveolar Type II Cells,Mol. Cell Biochem. 115, 79–84.

    PubMed  CAS  Google Scholar 

  23. Ando, Y., Imamura, S., Hong, Y.M., Owada, M.K., Kakunaga, T., and Kannagi, R. (1989) Enhancement of Calcium Sensitivity of Lipocortin I in Phospholipid Binding Induced by Limited Proteolysis and Phosphorylation at the Amino Terminus as Analyzed by Phospholipid Affinity Column Chromatography,J. Biol. Chem. 264, 6948–6955.

    PubMed  CAS  Google Scholar 

  24. Schlaepfer, D.D., and Haigler, H.T. (1990) Expression of Annexins as a Function of Cellular Growth State,J. Cell Biol. 111, 229–238.

    Article  PubMed  CAS  Google Scholar 

  25. Clark, D.M., Moss, S.E., Wright, N.A., and Crumpton, M.J. (1991) Expression of Annexin VI (p68, 67 kDa-calelectrin) in Normal Human Tissues: Evidence for Developmental Regulation in B-and T-Lymphocytes,Histochemistry 96, 405–412.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, Division of Neonatology, University of Wisconsin Perinatal Center, Meriter Hospital-Park, 202 South Park Street, 53715, Madison, WI

    Francis H. C. Tsao, Xiaoru Chen & Xiaoming Chen

  2. Department of Pathology, Northwestern University, 60611, Chicago, Illinois

    Chung-hsin Ts'ao

Authors
  1. Francis H. C. Tsao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaoru Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoming Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chung-hsin Ts'ao
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Tsao, F.H.C., Chen, X., Chen, X. et al. Annexin I in female rabbit reproductive organs: Varying levels in relation to maturity and pregnancy. Lipids 30, 507–511 (1995). https://doi.org/10.1007/BF02537024

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02537024

Keywords

Search

Navigation