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Enantiomeric resolution of diacylglycerol derivatives by high-performance liquid chromatography on a chiral stationary phase at low temperatures

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Lipids

Abstract

Separation of 1,2-diacylglycerol (DG) enantiomers as their 3,5-dinitrophenylurethane derivatives by high-performance liquid chromatography on a chiral column (Sumichiral OA-4100) was significantly improved at low temperature,e.g., at −30°C. A linear relationship between logarithmic retention volumes and the number of carbons and olefinic bonds of the DG enantiomers was obtained. The effects of temperature on the separation are discussed.

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Abbreviations

CSP:

chiral stationary phase

DG:

diacylglycerol

3,5-DNPU:

3,5-dinitrophenylurethane

HPLC:

high-performance liquid chromatography

Vr:

corrected retention volume

α:

separation factor

Rs:

peak resoltuion

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Fisheries, Hokkaido University, 041, Hakodate, Japan

    Toru Takagi & Toshiyuki Suzuki

Authors
  1. Toru Takagi
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  2. Toshiyuki Suzuki
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Takagi, T., Suzuki, T. Enantiomeric resolution of diacylglycerol derivatives by high-performance liquid chromatography on a chiral stationary phase at low temperatures. Lipids 28, 251–253 (1993). https://doi.org/10.1007/BF02536648

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  • DOI: https://doi.org/10.1007/BF02536648

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