Abstract
We investigated the effect of fish oil supplementation on the synthesis of prostaglandin E (PGE)in vivo by measuring the excretion of its catabolite, PGE-M, in 24-hr urine by gas chromatography/mass spectrometry. Forty healthy male volunteers (24–57 years of age) consumed a controlled basal diet providing 40% of energy from fat (P/S ratio about 0.8∶1), 130 mg/1000 kcal cholesterol, and a minimum of 22 mg/day of α-tocopherol (α-T), for three experimental periods lasting a total of 28 weeks. During period 1 (10 weeks) the diet was supplemented with placebo (PO) capsules (15×1 g/day) consisting of a blend of fats approaching the fatty acid profile of the basal diet. This was followed by a second 10-week period during which the subjects received 15×1 g/day capsules of fish oil concentrate (FOC). During period 3 (8 weeks) they continued the 15 g/day intake of FOC but received an additional 200 mg/day of α-T. PO and FOC capsules contained 1 mg α-T/g fat as antioxidant. A 14% reduction of PGE-M excretion was observed after 10 weeks of FOC supplementation (period 2), compared to an identical period of placebo supplementation (period 1), P=0.009. PGE-M excretion during the last week of period 3 was not significantly different from that at the end of period 2. The reduction in PGE synthesis in response to the relatively high marine oil supplementation was large in many subjects participating in this study.
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Abbreviations
- α-T:
-
α-tocopherol
- EPA:
-
eicosapentaenoate
- FOC:
-
fish oil concentrate
- GC/MS:
-
gas chromatography/mass spectrometry
- PGE:
-
prostaglandin E
- PGE-M:
-
11α-hydroxy-9,15-dioxio-2,3,4,5,20-pentanor-19-carboxyprostanoic acid
- PGI2 :
-
prostacyclin
- PO:
-
placebo oil
- P/S:
-
ratio dietary linoleate to saturated fatty acids
- PUFA:
-
polyunsaturated fatty acids
- TXA2 :
-
thromboxane A2
References
Bang, H.O., and Dyerberg, J. (1980)Dan. Med. Bull. 27, 202–205.
Kromhout, D., Bosschieter, E.B., and de Lezenne Coulander, C. (1985)N. Engl. J. Med. 312, 1205–1209.
Knapp, H.R., Reilly, I.A.G., Alessandrini, P., and FitzGerald, G.A. (1986)N. Engl. J. Med. 314, 937–942.
Knapp, H.R., and FitzGerald, G.A. (1989)N. Engl. J. Med. 320, 1037–1043.
Dyerberg, J. (1986)Nutr. Rev. 44, 125–134.
Fischer, S., Weber, P.C., and Dyerberg, J. (1986)Prostaglandins 32, 235–241.
Hornstra, G., Haddeman, E., Kloeze, J., and Verschuren, P.M. (1983) inAdvances in Prostaglandin Thromboxane and Leukotriene Research (Samuelsson, B., Paoletti, R., and Ramwell, P., eds.) Vol. 12, pp. 193–202, Raven Press, New York.
Fischer, S., and Weber, P.C. (1983)Biochem. Biophys. Res. Commun. 116, 1091–1099.
Fischer, S., and Weber, P.C. (1984)Nature 307, 165–168.
Fischer, S., and Weber, P.C. (1985)Biomed. Mass. Spectrom. 12, 470–476.
Takahashi, H., and Buñag, R.D. (1981)Hypertension 3, 426–432.
Hintze, T.H., and Kaley, G. (1985) inProstaglandins, Leukotrienes and Lipoxins (Bailey, J.M., ed.) pp. 321–332, Plenum Press, New York.
Karmali, R.A. (1980)Prostaglandins and Medicine 5, 11–28.
Narisawa, T., Takahashi, M., Niwa, M., Fukaura, Y., and Wakizaka, A. (1987)Gann 78, 791–798.
Minoura, T., Takata, T., Sakaguchi, M., Takada, H., Yamamura, M., Hioki, K., and Yamamoto, M. (1988)Cancer Res. 48, 4790–4794.
Goodwin, J.S., and Ceuppens, J. (1983)J. Clin. Immunol. 3, 295–315.
Field, W.E., II, Ferguson, F.G., Reddanna, P., and Reddy, C.C. (1988)Prostaglandins 35, 411–419.
ElMasry, M.N., and Rich, R.R. (1989)J. Clin Invest. 83, 1436–1440.
Hamberg, M., and Samuelsson, B. (1971)J. Biol. Chem. 246, 6713–6721.
Seyberth, H.W., Sweetman, B.J., Frölich, J.C., and Oates, J.A. (1976)Prostaglandins 11, 381–397.
Committee on Dietary Allowances, Food and Nutrition Board, Commission on Life Sciences, National Research Council (1980)Recommended Dietary Allowances, 9th edn., National Academy Press, Washington, D.C.
Ferretti, A., Flanagan, V.P., and Reeves, V.B. (1987)Anal. Biochem. 167, 174–180.
Ferretti, A., Flanagan, V.P., and Roman, J.M. (1985)Anal. Letters 18(B), 1571–1578.
Ferretti, A., Judd, J.T., Marshall, M.W., Flanagan, V.P., Roman, J.M., and Matusik, Jr., E.J. (1985)Lipids 20, 268–272.
Hornstra, G., and Stegen, J.C.H.C. (1989)Eicosanoids 2, 145–149.
Garrido, A., Garrido, F., Guerra, R., and Valenzuela, A. (1989)Lipids 24, 833–835.
Ferretti, A., Flanagan, V.P., and Reeves, V.B. (1988)Biochim. Biophys. Acta 959, 262–268.
Lote, C.J., and Haylor, J. (1989)Prostaglandins, Leukotrienes and Essential Fatty Acids 36, 203–217.
Lennon, E.A., and Poyser, N.L. (1986)Prostaglandins Leukotrienes and Med. 25, 71–89.
Lefer, A.M. (1985)Fed. Proc. 44, 275–280.
Lifschitz, M.D. (1981)Kidney Intl. 19, 781–785.
Pomerantz, K.B., and Hajjar, D.P. (1989)Arteriosclerosis 9, 413–429.
Brunda, M.J., Herberman, R.B., and Holden, H.T. (1980)J. Immunol. 124, 2682–2687.
Meydani, S.N., Yogeeswaran, G., Liu, G., Baskar, S., and Meydani, M. (1988)J. Nutr. 118, 1245–1252.
Baker, P.E., Fahey, J.V., and Munck, A. (1981)Cell. Immunol. 61, 52–61.
Chouaib, S., and Bertoglio, J.H. (1988)Lymphokine Res. 7, 237–245.
Pelus, L.M., and Strausser, H.R. (1977)Life Sci. 20, 903–914.
Taffett, S.M., and Russell, S.W. (1981)J. Immunol. 126, 424–427.
Williams, E.D., Karim, S.M., and Sandler, M. (1968)Lancet 1, 22–23.
Karmali, R.A., Marsh, J., and Fuchs, C. (1984)J. Natl. Cancer Inst. 73, 457–461.
Cameron, E., Bland, J., and Marcuson, R. (1989)Nutr. Res. 9, 383–393.
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Ferretti, A., Judd, J.T., Ballard-Barbash, R. et al. Effect of fish oil supplementation on the excretion of the major metabolite of prostaglandin E in healthy male subjects. Lipids 26, 500–503 (1991). https://doi.org/10.1007/BF02536593
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DOI: https://doi.org/10.1007/BF02536593