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Production of platelet-activating factor by human normodense and hypodense eosinophils

  • Cell Biology of PAF
  • Published:
Lipids

Abstract

Normodense eosinophils and neutrophils from normal donors produced considerable amounts of plateletactivating factor (PAF) when stimulated with ionophore A23187. PAF produced by eosinophils appeared to be degraded more rapidly than PAF formed by neutrophils, suggesting a higher activity of PAF-degrading enzyme in eosinophils. Substantial proportions of PAF newly formed by both eosinophils and neutrophils were shown to be cell-associated. By comparison, hypodense eosinophils obtained from a patient with idiopathic hypereosinophilic syndrome produced an extremely large amount of PAF and released much of it into the incubation medium. The accelerated formation of PAF in hypodense eosinophils may be related to various cardiovascular complications associated with hypereosinophilic syndrome.

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Abbreviations

BSA:

bovine serum albumin

CGP:

choline glycerophospholipids

EGP:

ethanolamine glycerophospholipids

GLC:

gas-liquid chromatography

GPC:

sn-glycero-3-phosphocholine

Hepes:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

HES:

hypereosinophilic syndrome

LTC4 :

leukotriene C4

PAF:

plateletactivating factor

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Teikyo University, 199-01, Sagamiko, Kanagawa, Japan

    Ayako Ojima-Uchiyama, Yasuo Masuzawa, Takayuki Sugiura & Keizo Waku

  2. Dokkyo University, School of Medicine, Mibu, Tochigi 321-02, Japan

    Takeshi Fukuda & Sohei Makino

Authors
  1. Ayako Ojima-Uchiyama
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  2. Yasuo Masuzawa
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  3. Takayuki Sugiura
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  4. Keizo Waku
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  5. Takeshi Fukuda
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  6. Sohei Makino
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Ojima-Uchiyama, A., Masuzawa, Y., Sugiura, T. et al. Production of platelet-activating factor by human normodense and hypodense eosinophils. Lipids 26, 1200–1203 (1991). https://doi.org/10.1007/BF02536531

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  • DOI: https://doi.org/10.1007/BF02536531

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