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Lipid abnormalities in pancreatic tissue of streptozotocin-induced diabetic rats

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Lipids

Abstract

Pancreatic lipid and fatty acid composition was determined in streptozotocin-induced diabetic rats and compared to control and insulin-treated diabetic rats. A pronounced decrease of total fatty acids was recorded and mainly accounted for by a drop of fatty acids in glycerides. Cholesterol, on the other hand, was significantly increased two- to three-fold, leading to an elevated cholesterol/phospholipid ratio. Morpho-cytochemical studies confirmed these findings because the multiple lipid droplets present in acinar cells of diabetic animals were found to be of cholesterol nature. The major alterations in phospholipid-fatty acid composition were characterized by an increase of linoleate coupled to a decrease of monounsaturates and arachidonate, suggesting defective metabolism of saturates and of linoleate. This was further supported by fatty acid ratios that suggested low Δ5 and Δ9 desaturation. Daily administration of insulin for 10 days restored and overcorrected the various lipid alterations. This study suggests that there are alterations in lipid composition and metabolism in the exocrine pancreas of chronic streptozotocin-induced diabetic rats. Their possible role in the mechanism regulating pancreatic function and secretion remains to be elucidated.

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Abbreviations

CE:

cholesterol ester

DG:

diglyceride

EDTA:

ethylenediamine tetraacetic acid

FA:

fatty acid

FC:

free cholesterol

HDL:

high density lipoprotein

MG:

monoglyceride

LDL:

low density lipoprotein

PL:

phospholipid

TC:

total cholesterol

TG:

triglyceride

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Authors and Affiliations

  1. Departments of Nutrition, Anatomy and Gastroenterology, University of Montreal and Research Center, Ste-Justine Hospital, Montreal, Quebec, Canada

    Emile Levy, Claude C. Roy, Guy Lepage & Moise Bendayan

Authors
  1. Emile Levy
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  2. Claude C. Roy
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  3. Guy Lepage
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  4. Moise Bendayan
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Levy, E., Roy, C.C., Lepage, G. et al. Lipid abnormalities in pancreatic tissue of streptozotocin-induced diabetic rats. Lipids 23, 771–778 (1988). https://doi.org/10.1007/BF02536220

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  • DOI: https://doi.org/10.1007/BF02536220

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