Abstract
This study tests the hypothesis that the rat chylomicrons are assembled and released into lymph similarly regardless of the site (rough or smooth endoplasmic reticulum) or pathway (phosphatidic acid or monoacylglycerol) of triacylglycerol biosynthesis. For this purpose we determined the lipid class, fatty acid and molecular species composition of the choline, ethanolamine, inositol and serine phospholipids of lymph chylomicrons during absorption of menhaden, mustard-seed and corn oil (monoacylglycerol pathway) or the corresponding fatty acid methyl or ethyl esters (phosphatidic acid pathway). The dietary fatty acids were found to be incorporated to various extents into different phospholipid classes, the proportions of which were not affected by the nature of the dietary fat. The chylomicron phospholipids contained 80–82% choline, 8% ethanolamine and 2.5% inositol glycerophospholipids, and much smaller amounts of serine and other minor phospholipids. Administration of a meal of each dietary fat resulted in a retention of approximately 50% endogenous fatty acids in the major glycerophospholipids of the chylomicrons. A minimum of 50% of the molecular species of the choline and ethanolamine glycerophospholipids contained at least one exogenous fatty acid. No significant discrepancies were found in the fatty acid and molecular species composition of the glycerophospholipids between chylomicrons from the oil and corresponding ester feeding. It is concluded that the chylomicrons arising from the monoacylglycerol (oil feeding) and the phosphatidic acid (ester feeding) pathways of triacylglycerol biosynthesis become enveloped in surfactant monolayers containing qualitatively and quantitatively identical classes and molecular species of phospholipids.
Similar content being viewed by others
Abbreviations
- GLC:
-
gas-liquid chromatography
- GPC:
-
choline glycerophospholipid
- GPE:
-
ethanolamine glycerophospholipid
- HDL:
-
high density lipoprotein
- HPLC:
-
high-performance liquid chromatography
- LPC:
-
lysophosphatidylcholine
- LPE:
-
lysophosphatidylethanolamine
- MG:
-
monoacylglycerol
- PA:
-
phosphatidic acid
- PC:
-
phosphatidylcholine
- PE:
-
phosphatidylethanolamine
- PI:
-
phosphatidylinositol
- PS:
-
phosphatidylserine
- SPH:
-
sphingomyelin
- TMS:
-
trimethylsilyl
- TLC:
-
thin-layer chromatography
- VLDL:
-
very low density lipoprotein
References
Yang, L.Y., Kuksis, A., and Myher, J.J. (1990)Inform 1, 329.
Clark, B., and Hubscher, G. (1961)Biochim. Biophys. Acta. 46, 479–494.
Clark, B., and Hubscher, G. (1960)Nature 185, 35–37.
Johnston, J.M. (1978) inDisturbances in the Lipid and Lipoprotein Metabolism (Dietschy, J.M., Gotto, A.M. and Ontko, J.A. eds.) pp. 57–68, American Physiological Society, Washington, D.C.
Kuksis, A., and Manganaro, F. (1986) inFat Absorption, (Kuksis, A. ed.) Vol. 2, pp. 233–259, CRC Press, Boca Raton.
Francone, O.L., Kalopissis, A.D., and Griffaton, G. (1989)Biochim. Biophys. Acta 1002, 28–36.
Mansbach, II, C.M., Arnold, A., and Garrett, M. (1987)Am. J. Physiol. 253, G673-G678.
Yang, L.Y., and Kuksis, A. (1987)Biochem. Cell Biol. 65, 514–524.
Yang, L.Y., and Kuksis, A. (1991)J. Lipid Res. 32, 1173–1186.
Yang, L.Y., Kuksis, A., and Myher, J.J. (1990)Biochem. Cell Biol. 68, 480–491.
Myher, J.J., Kuksis, A., Yang, L.Y., and Marai, L. (1987)Biochem. Cell Biol. 65, 811–821.
Skipski, V.P., Peterson, R.F., and Barclay, M. (1964)Biochem. J. 90, 374–378.
Rouser, G., Fleischer, S., and Yamamoto, A. (1970)Lipids 5, 494–496.
Myher, J.J., and Kuksis, A. (1984)Biochim. Biophys. Acta 795, 85–90.
Myher, J.J., Kuksis, A., and Pind, S. (1989)Lipids 24, 396–407.
Myher, J.J., and Kuksis, A. (1982)Can. J. Biochem. 60, 634–650.
Myher, J.J., Kuksis, A., and Yang, L.Y. (1990)Biochem. Cell Biol. 68, 336–344.
Yang, L.Y., Kuksis, A., and Myher, J.J. (1990)Inform 1, 354.
Myher, J.J., Kuksis, A., and Pind, S. (1990)Inform 1, 332.
Shaikh, N.A., and Kuksis, A. (1982)Can. J. Biochem. 60, 444–451.
Redgrave, R.T. (1971)Aust. J. Exp. Biol. Med. Sci. 49, 209–224.
Nelson, G.J. (1967)Lipids 2, 323–328.
Van Meer, G., (1989)Annu. Rev. Cell Biol. 5, 247–275.
Arvidson, G.A.E., and Nilsson, A. (1972)Lipids 5, 344–348.
Patton, G.M., Clark, S.B., Fasulo, J.M., and Robins, S.J. (1984)J. Clin. Invest. 73, 231–240.
Scow, R.R., Stein, Y., and Stein, O. (1967)J. Biol. Chem. 242, 4919–4924.
Parthasarathy, E., Subbiah, P.V., and Ganguly, J. (1974)Biochem. J. 140, 503–508.
Mansbach, II, C.M. (1977)J. Clin. Invest. 60, 411–420.
Patton, G.M., Robins, S.J., Fasulo, J.M., and Clark, S.B. (1985)J. Lipids Res. 26, 1285–1293.
Higgins, J.A., and Fieldsend, J.K. (1987)J. Lipid Res. 28, 268–278.
Janero, D.R., and Lane, M.D. (1983)J. Biol. Chem. 258, 14496–14504.
Vance, J., and Vance, D.E. (1988)J. Biol. Chem. 263, 5898–5909.
Kawamoto, T., Akino, T., Nakamura, M., and Mori, M. (1980)Biochim. Biophys. Acta 619, 35–47.
Landin, B., and Nilsson, A. (1984)Biochim. Biophys. Acta 793, 105–113.
Vance, J.E., and Vance, D.E. (1986)J. Biol. Chem. 261, 4486–4491.
Yao, Z., and Vance, D.E. (1988)J. Biol. Chem. 263, 2998–3004.
Author information
Authors and Affiliations
About this article
Cite this article
Yang, LY., Kuksis, A. & Myher, J.J. Similarities in surface lipids of chylomicrons from glyceryl and alkyl ester feeding: Major components. Lipids 26, 806–818 (1991). https://doi.org/10.1007/BF02536162
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02536162