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The effect of dietary zinc deficiency on the lipid composition of the rat erythrocyte membrane

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Lipids

Abstract

The effect of dietary zinc deficiency in the rat on the lipid composition of the erythrocyte membrane was determined. Weanling male Wistar rats were fed an egg whitebased diet containing <1.0 mg Zn/kg dietad libitum. Control rats were either pair-fed orad libitum-fed the basal diet suppelemented with 100 mg Zn/kg diet. A zinc refed group was fed the −Zn diet until day 18 and then pairfed the +Zn diet until day 21. The voluntary feed restriction associated with dietary zinc deficiency resulted in erythrocyte membranes that had depressed phospholipid/protein and elevated cholesterol/phospholipid ratios. Similarly, all feed restricted groups had elevated 22-carbon n−3 polyunsaturated fatty acid (PUFA) and depressed 22-carbon n−6 PUFA concentrations in alkenylacyl and diacyl glycerophosphoethanolamine, phosphatidylserine and phosphatidylcholine; they also had depressed 24∶2n−6 levels in sphingomyelin. The relative concentrations of phospholipids in the membrane was similar between −Zn and +Zn (ad libitum) groups; however, the −Zn group had significantly less phosphatidylserine relative to +Zn (pair-fed) controls.

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Abbreviations

ANS:

8-anilinonaphthalene-1-sulfonic acid

FAME:

fatty acid methyl ester

FFA:

free fatty acids

GPE:

glycerophosphoethanolamine

LPC:

lysophosphatidylcholine

MCV:

mean corpuscular volume

MECL:

modified equivalent chain length

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

PUFA:

polyunsaturated fatty acids

SPH:

sphingomyelin

TLC:

thin-layer chromatography

−Zn:

zinc deficient

+ZnAL:

zinc-adequate,ad libitum

+ZnPF:

zinc-adequate, pair-fed, pair-fed

+ZnRF:

zinc refed

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Authors and Affiliations

  1. Department of Nutritional Sciences, College of Biological Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Eric R. Driscoll & William J. Bettger

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  1. Eric R. Driscoll
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Driscoll, E.R., Bettger, W.J. The effect of dietary zinc deficiency on the lipid composition of the rat erythrocyte membrane. Lipids 26, 459–466 (1991). https://doi.org/10.1007/BF02536073

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  • DOI: https://doi.org/10.1007/BF02536073

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