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Erucic acid-induced alteration of cardiac triglyceride hydrolysis

  • Published:
Lipids

Abstract

Male Wistar rats were fed for 3 or 10 days with high erucic acid rapeseed oil (HEAR) or trierucate (TE). These diets produced increased myocardial triglyceride (TG) levels. Cardiac lipid accumulation was related to basal-and hormone- (glucagon, norepinephrine) stimulated lipolysis, determined as glycerol release, which proved to be enhanced in isolated, perfused hearts from HEAR- and TE-fed rats. Endogenous TG levels in isolated hearts from rats fed the stock and the sunflowerseed oil (SSO) diet were low and probably rate-limiting for tissue lipolytic activities. HEAR feeding of rats did not modify the rate of erucic acid (22∶1) oxidation in heart. Prolonged HEAR and TE feeding led to a decrease in the endogenous TG level, a process in which the increased rate of TG hydrolysis might play an important role. The enhanced breakdown of tissue TG in hearts from TE-and HEAR-fed rats was accompanied by an increased release of fatty acids into the coronary effluent. Erucic acid was a major constituent of the perfusate fatty acids. Evidence is presented that the site of the intracellular TG breakdown is associated with lysosomes, since a subcellular fraction enriched in acid lipase, N-acetyl-β-glucosaminidase and TG could be isolated from heart homogenates of TE-fed rats. Fatty acids seemed to be an important regulator of tissue lipase activity: palmitate inhibited glucagon-stimulated lipolysis, which suggests the tissue lipase is subject to product inhibition by fatty acids.

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Authors and Affiliations

  1. Department of Biochemistry 1 Medical Faculty, Erasmus University Rotterdam, PO Box 1738, 3000-DR, Rotterdam, The Netherlands

    Hans Stam, Tiny Geelhoed-Mieras & Willem C. Hülsmann

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  1. Hans Stam
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  2. Tiny Geelhoed-Mieras
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  3. Willem C. Hülsmann
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Stam, H., Geelhoed-Mieras, T. & Hülsmann, W.C. Erucic acid-induced alteration of cardiac triglyceride hydrolysis. Lipids 15, 242–250 (1980). https://doi.org/10.1007/BF02535834

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  • DOI: https://doi.org/10.1007/BF02535834

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