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Cholesteryl ester analogs inhibit cholesteryl ester but not triglyceride transfer catalyzed by the plasma cholesteryl ester-triglyceride transfer protein

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Lipids

Abstract

A lipid transfer protein complex (LTC), purified from human plasma by immunoaffinity chromatography, catalyzed the interlipoprotein transfer of cholesteryl esters (CE) and triglycerides (TG). The CE transfer activity of LTC was governed by the strucutre of the CE. Incubation of LTC with long chain CE both activated and stabilized LTC. Short chain CE also enhanced the CE and TG transfer activity of LTC during the initial time of incubation. However, LTC's incubation with short chain CE induced a subsequent and time-dependent loss of CE transfer activity without concomitant loss of TG transfer activity. The data indicate that the CE and TG transfer activity of LTC can be reglated independently.

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Abbreviations

BSA:

bovine serum albumin

CA:

cholesteryl acetate

CAB:

cholesteryl acetylbromide

CE:

cholesteryl ester

CETP:

cholesteryl ester transfer protein

CL:

cholesteryl linoleate

CO:

cholesteryl oleate

HDL:

high density lipoproteins

HPLC:

high performance liquid chromatography

LDL:

low density lipoproteins

Lp:

lipoproteins

LTC:

lipid transfer protein complex

NMR:

nuclear magnetic resonance

TG:

triglyceride

TLC:

thin-layer chromatography

TMS:

trimethyl silane

VLDL:

very low density lipoproteins

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Author notes

  1. Steven J. Busch

    Present address: Merrell Dow Research Institute, 2110 E. Galbraith Road, 45215, Cincinnati, OH

Authors and Affiliations

  1. Division of Lipoprotein Research, Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 45267-O575, Cincinnati, OH

    Steven J. Busch & Judith A. K. Harmony

Authors
  1. Steven J. Busch
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  2. Judith A. K. Harmony
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Busch, S.J., Harmony, J.A.K. Cholesteryl ester analogs inhibit cholesteryl ester but not triglyceride transfer catalyzed by the plasma cholesteryl ester-triglyceride transfer protein. Lipids 25, 216–220 (1990). https://doi.org/10.1007/BF02535750

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  • DOI: https://doi.org/10.1007/BF02535750

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